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Danny Newman Danny Newman

Melanospora cf. lagenaria on old, rotting, fallen

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Johan Boonefaes Johan Boonefaes

Unknown anamorph found on the ground in coastal sa

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Hardware Tony Hardware Tony

Small clavate hairs, negative croziers and IKI bb

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Danny Newman Danny Newman

indet. Rutstroemiaceae sp. on unk. fallen leavesMc

15-12-2025 21:47

Pol Debaenst

Good evening, On 12/11/2025 I found ascomycetes w

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Danny Newman Danny Newman

Pseudosclerococcum golindoi (det: Zotto)near Cosb

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Danny Newman Danny Newman

ITS sequences from the following two collections B

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Danny Newman Danny Newman

indet. Rhytismataceae on oak leafnear Purchase Roa

09-12-2025 12:06

Andgelo Mombert Andgelo Mombert

Bonjour,Je recherche l'article concernant Hypobryo

13-12-2025 17:26

Buckwheat Pete

Hello everyone,I have a rather interesting ascomyc

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Petit asco indéterminé
François Freléchoux, 25-08-2025 17:37
François FreléchouxBonjour,
Nous avons trouvé samedi dernier à l'étage alpin à Ovronnaz (VS), région du Grand Muveran, un petit ascomycète stipité dans une combe à neige (Salicion herbaceae), en présence de Saxifraga aizoon, Soldanella alpina, Vaccinium vitis-idaea, Salix herbacea.
Macro. Cupule 1-6 mm de diamètre, 2-4 mm de hauteur, brune à marge un peu plus foncée. Pied jusqu'à 12 mm de long x 0,5 mm de diamètre, sinueux, noir en haut, incolore en bas. Il ne semble pas pourvu d'un sclérote et semble lié aux mousses.
Micro. Asques I+, 140-160 µm x 9-11 µm. Spores 14-17 x 6-7,5 µm, fusiformes et semblent amyloïdes (au moins sur une extrémité). Paraphyses env. 110-140 x 2-3,5 µm, un peu élargie à l'extrémité qui semble pourvue de matière amorphe. Excipulum ectal avec cellules rondes. Il semble y avoir dans la chair des structures produisant des conidies.
Auriez-vous une idée du genre ou de l'expèce ?
Plus important encore : Auriez-vous de la littérature à me proposer ?
Merci d'avance
François
  • message #83359
  • message #83359
  • message #83359
Hans-Otto Baral, 26-08-2025 08:51
Hans-Otto Baral
Re : Petit asco indéterminé
You do not have the fungus fresh? Important would be the nuclear number in the spores and possible VBs in the paraphyses.

I am a bit in doubt that the apos are connected to moss. Usually there is some remnants of mummified plant substrate or a sclerotium. 

Surely the spores are not amyloid.

A possibility is Sclerotinia trifoliorum, did you look if 4 spores are smaller in the ascus than the other 4?
François Freléchoux, 26-08-2025 09:01
François Freléchoux
Re : Petit asco indéterminé
Hi Zotto,

I have already dried the samples. They will be sequenced. I still have one fresh sample.

I found a rhizome of a phanerogam (Saxifraga aizoides) in the moss, possibly linked to the fungus.

I took a lot of microphotos. I will send them to you privately soon.
Best regards,
François
Hans-Otto Baral, 26-08-2025 09:21
Hans-Otto Baral
Re : Petit asco indéterminé
Please don't overload me with pictures. If they are in Congo like the below one it does not help me much. A mature ascus with spores would be important at first to see if it is S. trifoliorum. When you look again at your fresh apo, please take water and do not press on the slip.
François Freléchoux, 26-08-2025 09:39
François Freléchoux
Re : Petit asco indéterminé
Ok, I'll observe matures spores in the water. And indeed, in several asci, the two sets of four spores are clearly different in size.
François Freléchoux, 27-08-2025 10:16
François Freléchoux
Re : Petit asco indéterminé
I looked at the spore nuclei. It seems to me that there are several, but their outlines are unclear. On the other hand, I checked the ectal excipulum, which is indeed textura intricata (for Sclerotinia).
François Freléchoux, 27-08-2025 10:18
François Freléchoux
Re : Petit asco indéterminé
The material will be sequenced by A. Gross at the WSL.
Hans-Otto Baral, 27-08-2025 11:50
Hans-Otto Baral
Re : Petit asco indéterminé
Sclerotinia has an ectal excipulum of t. globulosa. The large spores of S. trifoliorum are 4-nucleate. This species exists several times in GenBank (ITS), e.g. EU082465, with 98.8-100% similarity, but some of them are misidentified as S. sclerotiorum.