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13-04-2024 21:10

B Shelbourne B Shelbourne

• Genus Cistella on dicots: Habitat, macro, hair

08-04-2024 19:57

Elisabeth Stöckli

Bonsoir,Récolté au bord du chemin, apothécie 0.

15-04-2024 16:09


Bonjour,Trouvé cet ascomycète sur vieille bouse

07-04-2024 20:49

B Shelbourne B Shelbourne

Another species that appears easy to identify from

14-04-2024 10:31

Castillo Joseba Castillo Joseba

Me mandan el material seco de Galicia (España) re

13-04-2024 12:11

Karen Poulsen

Hi I found these under loose bark on a fallen bra

13-04-2024 12:49

Karen Poulsen

On standing stem from last year. Surrounded by hyp

13-04-2024 17:39

Karen Poulsen

Hi, On Myxarium on the cutting surface of tall st

18-10-2022 01:37

Zuidland Peter

I need help with this one please.Vast numbers of t

12-04-2024 12:22

B Shelbourne B Shelbourne

Orbilia xanthostigma, sensu Baral (2020). • Hab

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Cistella on dicot
B Shelbourne, 13-04-2024 21:10
B Shelbourne• Genus Cistella on dicots: Habitat, macro, hairs, and spores.
• Possibly C. rubrotincta (from Zotto's folders): Spore size, long and tapering hairs.

Habitat: On dead, hollow, standing stems of herbaceous plant, 2-3 cm wide, lower part of stem but not base, damp when found, not seen on several other stems nearby, in a clearing, no bodies of water in the immediate vicinity, mixed deciduous woodland, mostly Betula pendula and Quercus robur, Low Weald, England (south), mid-April, after rain.

Associates: Lots of small brownish conidiophores, and a few larger black apothecia – possibly Pyrenopeziza (seems to be a common associate).

Apothecia: 20+ on the stem, in a loose group, generally scattered but occasionally 2-caespitose, 0.2-0.3 (0.5) mm diameter without hairs (< 0.6 mm with hairs), < 0.1 mm high (1.5 mm with hairs), pale yellowish with a hint of pink (apricot), shallow cupulate to discoid, translucent, gelatinous appearance and texture; margin distinct, more in-rolled until maturity, like receptacle; hairs whitish, more abundant and longer at the margin, when immature thick and white, when mature appearing conglutinated in regular groups, and pointing outwards; disk more translucent, greyish due to dark substrate, gelatinous appearance, eventually convex.

Storage and method: A short piece of stem stored overnight in a damp box, sections taken from a single large mature-looking apothecium, mounted in tap water (unfortunately median section was a little thick and turned hymenium down), a little pressure applied to separate cells of the hymenium, IKI added to water mount, KOH + CR added after IKI.

IKI: Apical rings weakly bb, some dextrinoid ascoplasm, hairs with yellow contents.
KOH + CR: Hairs strongly red, gel at apices dissolving, ornamentation remaining; exudate more greenish.

Asci: Cylindrical to mildly clavate, apex conical, IKI bb, croziers, 1-2-seriate, many discharging in water mount,.
• Living mature: ~ 33-45 x 4.5-5 (5.7) um, pars sporifera ~30-40%, apex more rounded-conical, spores diagonally oriented, one spore at apex, sometimes with hyaline to greenish VBs above base of ascus.
• Dead mature: ~30-38 x 3.5-4.5 um, pars sporifera ~60-90%, apex more acute-conical, small apical thickening ~1 um, spores more vertically oriented
• Discharged: Small poricidal opening, cylindrical, apex acute, usually with some amyloid material remaining at the extreme apex but once without, still relatively turgid, many small LBs.

Spores: Narrow-ellipsoid to fusiform, poles rounded to acute, usually heteropolar with the base more attenuated and acute (apex more rounded-obtuse), a few tiny shadowy LBs around the poles, OCI 1.
Vital spores measured in water mount: (6.9) 7.1-8.0 (8.7) × 1.7-2.1 µm, Q = (3.5) 3.6 - 4.2, n = 11, mean = 7.5 × 1.9 µm, Q mean = 3.9.

Paraphyses: Gradually lanceolate to filiform, 2.5-3 um wide, apex rounded-acute, ~3 septa, exceeding immature asci, very faint yellowish VBs (very lowly refractive and more noticeable at lower magnification), several shadowy LBs more noticeable in KOH + CR.

Marginal hairs: ~40-60 x 5-6 um, apices ~2.5-3 um wide, abundant at margin, some at flank, broad-cylindrical, tapering, sinuous, apex rounded, protruding part observed with 3-4 septa, 1-2 apical cells with very light ornamentation of tiny warts, ornamentation remaining in KOH, longer hairs with tangled apices, apparently conglutinated, bases apparently with VBs like paraphyses; some shorter hairs, 30-40 um long, clavate apical cell, apparently unornamented.

Exudate: Yellow-brown, thick, globular, gelatinous, on surface of ectal excipulum and short hairs around base.

Ectal ex: Very light yellowish at low magnification, hyaline at high magnification, surface with textura prismatica, internally possibly textura globosa.

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B Shelbourne, 15-04-2024 13:30
B Shelbourne
Re : Cistella on dicot
I think the stem could be Apiaceae, so with the spore size I'm wondering about C. grevillei.
Michel Hairaud, 15-04-2024 20:07
Michel Hairaud
Re : Cistella on dicot
Hi , that's also my guess 
B Shelbourne, 16-04-2024 10:45
B Shelbourne
Re : Cistella on dicot
Thank you for looking.

This is my first Cistella sp., and some of the smaller spored species on dicots look quite similar. I didn't find any accessible key and I'm not sure about the substrate preferences, but I looked through Zotto's folder.

I did see the paper of Quijada et al. (2015) on Hyphodiscus and Cistella in the Canary Islands. Based on their suggestions (following Raitviir), the length of the spores and asci seem to fit better with C. hungarica, but I didn't see this species in Zotto's folder.

B Shelbourne, 16-04-2024 10:54
B Shelbourne
Re : Cistella on dicot
I see now that these are synonymised in Zotto's folder. That is good to see because the spore sizes reported in Zotto's folder are quite variable, and some of those I measured are a little too long compared to observations of Quijada et al.