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27-01-2026 11:43

Malcolm  Greaves Malcolm Greaves

Is anyone with experience of DNA testing able to t

25-01-2026 16:08

Malcolm  Greaves Malcolm Greaves

This Geoglossum had spores mostly 70-80 (87) with

26-01-2026 11:49

Margot en Geert Vullings

We found this possible anamorph on a dead Cytisus

25-01-2026 23:23

Tomaz Vucko Tomaz Vucko

Hello! I found this species that resembles Delitsc

18-01-2026 12:24

Josep Torres Josep Torres

Hello.An anamorph located on the surface of a thin

23-01-2026 21:50

Cameron DK

I am looking for this please publication. is anyon

10-01-2026 20:00

Tom Schrier

Hi all,We found picnidia on Protoparmeliopsis mur

21-01-2026 19:55

Bohan Jia

Hi,  Could this be Nemania aureolutea? Or did I

21-01-2026 16:32

Gernot Friebes

Hi,I need your help with some black dots on a lich

21-01-2026 16:48

Gernot Friebes

Hi,after my last unknown hyphomycete on this subst

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DNA match
Malcolm Greaves, 27-01-2026 11:43
Malcolm  GreavesIs anyone with experience of DNA testing able to tell me if a 97% match to a know species is enough to justify giving it that name or is it likely to be a related but different species?
Thanks
Mal
Nicolas VAN VOOREN, 27-01-2026 11:52
Nicolas VAN VOOREN
Re : DNA match
Well, you cannot answer by yes or no to such a question. It depends of what type of sequence (locus) you compare, length of these sequences, quality of your sequence, etc.
In the best case, with 97% you can hypothesize this is a different 'species', but to be sure, you must generate a phylogeny and also evaluate the morphological differences.
Hans-Otto Baral, 27-01-2026 12:02
Hans-Otto Baral
Re : DNA match
Assuming that you have an ITS sequence, the idea of 97% similarity resp. 3% distance is antiquated. In some groups of fungi this works, in others you need to accept lower distances, such as 1.5-2%, and in some there is no difference at all in the ITS between species and you must use other regions. To evaluate an ITS distance you must search for ATCATTA and TGACCT in your sequence and only test the part between them. When you got the distance in GenBank Blast you must look how many gaps are among the differences, because these count less than nucleotide differences. What kind of fungus do you have?