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25-03-2023 14:42

Edvin Johannesen Edvin Johannesen

I have received this dried specimen for microscopy

29-03-2023 13:05

Georges Greiff

Bonsoir,I would like to please request the followi

28-03-2023 19:16

Enrique Rubio Enrique Rubio

J'aimerais connaître la revision de Van Vooren su

28-03-2023 01:34

Stefan Jakobsson

On wood of a still fairly hard trunk of spruce I f

28-03-2023 11:04

Margot en Geert Vullings

Found this Lichenoptella (Trichothyrina) on Juncus

27-03-2023 00:36

Juuso Äikäs

A finding from last fall: a plentiful amount of lo

17-11-2014 15:55

Salvador Tello

Hola a todos.Tengo este hongo que he cogido crecie

24-03-2023 16:08

Bernard Declercq Bernard Declercq

Hello,A friend of mine collected galls with pynidi

24-03-2023 12:53

Riet van Oosten Riet van Oosten

Hello, Saw this strange ascus Schizoxylon composi

25-03-2023 09:04

Bernard Declercq Bernard Declercq

Hello,I am looking for following paper:Motohashi K

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Whitish inoperculate on the liverwort Aneura pinguis
Edvin Johannesen, 25-03-2023 14:42
Edvin JohannesenI have received this dried specimen for microscopy. In the fresh state the apothecia are (purplish-greyish) white, but they become black in the dry state (see photos of both states). On rehydration they appear somewhat gelatinous, dark purplish brown.
Hymenium is a brown, dense palisade. Excipulum is paler brown, I would say textura epidermoidea (?). Asci and spores appear dead. Mature spores are brown, 20 x 6 micr., ellipsoid/allantoid, aseptate or I suspect becoming 1-septate (hard to see).  Asci IKI negative. 
All micro images in water.
I have not been able to find any bryophilous inoperculates with such brown spores.

Any suggestions?
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Georges Greiff, 29-03-2023 13:03
Re : Whitish inoperculate on the liverwort Aneura pinguis
Very interesting collection. I have not seen any inoperculates on liverworts with brown spores. It may well be undescribed. Bryoscyphus is superficially similar and seeing living paraphyses would have helped. Bryoscyphus conocephali agg. is on liverworts in similar habitats. It is not that but it might be related.

Edvin Johannesen, 29-03-2023 14:55
Edvin Johannesen
Re : Whitish inoperculate on the liverwort Aneura pinguis
Thanks for your opinion, Georges.  I will have another attempt at looking for paraphyses (I doubt they will be alive). I assume that the spore color is not a result of necrosis or some other process?
Georges Greiff, 29-03-2023 15:19
Re : Whitish inoperculate on the liverwort Aneura pinguis
Dear Edvin,

I have not noted brownish spores in any of the herbarium material of Bryoscyphus that I have examined, but that doesn't mean it won't happen. The darkening of the dry, old apothecia is a common occurrence Bryoscyphus, including in B. conocephali I collected recently. Perhaps this pigment has gone into the spores. I can check if this darkening happens in spores of my B. conocephali.

Unfortunately the paraphyses will be dead so you won't see the VBs. Perhaps now only sequencing can place this collection with more certainty within one of the bryophilous Leotiomycete groups. They are tricky even when fresh.

All the best,
Edvin Johannesen, 29-03-2023 15:40
Edvin Johannesen
Re : Whitish inoperculate on the liverwort Aneura pinguis
It would be great if you could check this - thanks!