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17-04-2025 15:37

Nicolas VAN VOOREN Nicolas VAN VOOREN

Bonjour.Je suis à la recherche de collections (r

17-04-2025 13:23

Jan Eckstein

Hello,there is an interesting collection of a tiny

16-04-2025 08:53

Josep Torres Josep Torres

Hello.An anamorph photographed under holm oaks thi

15-04-2025 15:06

Francois Guay Francois Guay

I found this small white disco on decaying deciduo

13-04-2025 22:47

éric ROMERO éric ROMERO

Bonjour, Je sollicite votre avis pour cette colle

15-04-2025 21:39

Andreas Gminder Andreas Gminder

Dear all,struggeling as every year with the aquati

10-04-2025 10:09

Mathias Hass Mathias Hass

Hi, Found in March on last years stick from Rubus

09-04-2025 19:37

Miguel Ãngel Ribes Miguel Ángel Ribes

Good afternoon This 1-2 mm Mollisia was growing o

14-04-2025 23:19

Viktorie Halasu Viktorie Halasu

Hello, I've microscoped exsiccate of an Otidea wh

14-04-2025 15:11

Lennert Gees

Greetings!For my master's dissertation I work on c

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Calycina (?) sp.
éric ROMERO, 02-04-2024 16:24
éric ROMERO
Bonjour,

J'ai besoin de votre aide pour ce petit asco blanc.

Récolté sur bois de conifère (Pinus sylvestris probable) très dégradé, fortement humide et colonisé pas Chlorociboria sp., secteur tourbière du Waldeck (F-67) le 17/03/24.

Apothécies sessile, discoïdes, d'abord sphériques, puis à marge flexueuse, blanches, translucides, très fragiles, n'excédant pas 0,6mm, faiblement adhérentes au support.

Asques clavés, inoperculés, crochet +, bituniquées, IKI + (Bleu). 50-65 X 6,5-8µ.

Paraphyses cylindriques, glabres, extrémités arrondies, septées (peu et/ou irrégulièrement), guttulées, ne dépassant pas des asques.

Excipulum textura angularis-globulosa.

Poils filiformes, hyalins, pointus, peu nombreux et isolés.

Spores ellipsoïdes-oblongues, hyalines, septés (1 cloison +/- centrale, rarement 2 observées), parfois guttulées de manière variable (souvent plusieurs petites guttules ou parfois 1 guttule moyenne par segment). (7,1) 8-10,5 (11) X 2,7-3,8µ.

Merci d'avance pour vos commentaires, éric.
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Hans-Otto Baral, 02-04-2024 16:47
Hans-Otto Baral
Re : Calycina (?) sp.
The hairs are important, woh long are they and are they always such tapering? Never glassy at the top?

These guttules in excipulum and paraphyses occur e.g. in Hyaloscypha vitreola but also in Mimicoscypha (previously Phialina) lacrimiformis and apparently in Resinoscypha variepilosa (not sure). But all have rather long hairs and longer or narrower spores.
éric ROMERO, 02-04-2024 22:45
éric ROMERO
Re : Calycina (?) sp.
Bonjour Zotto,

J'ai encore de la matière donc j'ai repris 5 ou 6 exemplaires pour refaire des images des poils et les mesurer. Ils sont lisses, pointus à légèrement arondis, plus épais vaers la base. Les mesures sont comprises entre 34 - 39 x 1,9 - 2,7, une dizaine de poils mesurés.

Après cette orientation et de nouvelles recherches, Hyaloscypha albohyalina serait-il un bon candidat ?

éric.
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Hans-Otto Baral, 03-04-2024 09:58
Hans-Otto Baral
Re : Calycina (?) sp.
My impression is that the spores are non-septate when inside living asci and septation occurs only after ejection or inside dead asci. But this should be verified. Hyaloscypha in the wide sense appears to me rather clear, but today some members of the genus were transferred to new genera, I remember H. paludosa is not Mimicoscypha paludosa.
éric ROMERO, 03-04-2024 16:03
éric ROMERO
Re : Calycina (?) sp.
Bonjour Zotto,

J'ai repris des exemplaires plus vieux et jaunissant, les spores septées sont bien à priori mortes.

J' ai refait les mesures (72 spores), et cela donne : (8,6) 8,9-12,9 X 2,8-4,3µ - OCI 3-4.

Cela nous fait-il avancer ? Un sequençage serait-il souhaitable ?

éric.
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Hans-Otto Baral, 03-04-2024 16:15
Hans-Otto Baral
Re : Calycina (?) sp.
So here you have purely non-septate spores. DNA would surely help, but the apos must be clean, dangerous is to use some which were several days in the fridge. I hope you have your photos also without piximeter, this method is quite disturbing and hides the spores, much better is a single scale for each photo and only a collective measurement.
éric ROMERO, 03-04-2024 16:43
éric ROMERO
Re : Calycina (?) sp.
La météo le permettant, les apos son dans le jardin. Par contre, pour les photos, je n'ai pas gardé celles sans les mesures..., je le ferai pour mes prochaines études.

Merci beaucoup pour l'aide apportée et les conseils, éric.
Kosonen Timo, 06-04-2024 14:24
Kosonen Timo
Re : Calycina (?) sp.
Hi,

This is an interesting sample. To me it looks very much like Mimicoscypha with the frequent guttules in excipulum and in the hairs especially. However, we have observed M. lacrimiformis both with and without guttules at the same locality / date and both later found 100% identical (sequence). M. lacrimiformis (the only Mimicoscypha sequenced) is a possibilty. M. mimica is another option as it also has crozier.

Resinoscypha (variepilosa) is yet another option. It too has guttules inside cells, spores usually <10 um long. Developing hairs bulky and not evenly tapering - proportionally wider compared to mature hairs that are more typical thin-walled multiseptate tapering etc. And it has cyanophilous substance ("resin") inside the hairs. Thereäs something non-Resinoscypha-like in the macro. 

I dont find these very easy. Resinoscypha is fairly common here Spring and Autumn like. Often I have thought I found Mimicoscypha only to find out based on sequence it is a Resinoscypha after all. Mimicoscypha possibly a Spring season species, but not enough n to really believe that myself.

It could be something else too... These hyaloscyphoids with multiseptate hairs have many homes :-)

Timo
Kosonen Timo, 06-04-2024 16:51
Kosonen Timo
Re : Calycina (?) sp.
sorry for the missing verbs, but maybe that was understandable still... or maybe no sense at all :-) :-) :-)

Timo
éric ROMERO, 06-04-2024 18:35
éric ROMERO
Re : Calycina (?) sp.
Hi Timo,

Thank you very much for your answer and all is goo life that.

I continue my research, the biggest difficulty is to find documentation. Didier Argaut will help me, and if it's necessary, we will sequence this species.

éric.