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On Populus sp. bark
Elsa Sousa, 04-02-2017 21:02
Hi,

I need some help here because I just don't know where to begin...

Ascomata ±102, dried, collected some months ago, almost one year.

Spores 27.8-35.2 x 5-6.5
Me 31.9x5.7     4(?)-7 septate
Ascus 115.6x17.76     
Paraphyses filiform some with slightly enlarged tips 


Thank you,
Elsa
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Hans-Otto Baral, 04-02-2017 21:14
Hans-Otto Baral
Re : On Populus sp. bark
This requires the iodine reaction of the ascus tip and a picture of the excipulum, in section or surface view. At present I have no idea. The substrate looks a bit lke wood, is it decayed bark, perhaps inner side of bark? On the moist ground?
Which country did you collect it?
Elsa Sousa, 04-02-2017 22:11
Re : On Populus sp. bark
Hi Otto, thank you for your answer

I was afraid somebody would ask me for iodine reaction...Mine finished, so... I could try with 2 options, iodopovidona 10% iodine, or one solution with 6 g iodine + 2g potassium iodide + etanol 70º /100ml. What do you think is the best?


About the collect: here in Portugal Braga. Wet place, inner bark of populus (fallen).


Elsa

Hans-Otto Baral, 04-02-2017 22:59
Hans-Otto Baral
Re : On Populus sp. bark
I think you can test the first one, maybe it works.
Elsa Sousa, 04-02-2017 23:04
Re : On Populus sp. bark
Sorry Otto,

I missed up all these...I was trying to spare the apos and choose another which were close. I thought they were the same, a bit more dried. So, I'm going to correct the macro photos, and add the blue tip of iodine reaction. Later I think about the other, definitely completely different.

Elsa
Hans-Otto Baral, 04-02-2017 23:12
Hans-Otto Baral
Re : On Populus sp. bark
With dead material it is very very complicated to work.
Elsa Sousa, 04-02-2017 23:30
Re : On Populus sp. bark
Otto, 

Sorry for my ignorance, but should dead material have still complete spores inside asci?

Thank you,
Elsa
Hans-Otto Baral, 04-02-2017 23:39
Hans-Otto Baral
Re : On Populus sp. bark
surely, but sometimes many spores get ejected during the drying process, and less mature spores remain in the asci. Anyway, you lose a lot of characters in the dead state.
Elsa Sousa, 04-02-2017 23:50
Re : On Populus sp. bark
Dear Otto,

Thank you for your patience. After some search, I think this is Durella connivens. 
https://www.asturnatura.com/fotografia/setas-hongos/durella-connivens-fr-rehm-2/2493.html

Do you agree?
Hans-Otto Baral, 05-02-2017 09:58
Hans-Otto Baral
Re : On Populus sp. bark
I actually thought about this species, but to affirm it you should simply put an apothecium upside down on a slide and press with the cober slip. Then you see the brown excipulum which must show a parallel (radial) hyphal structure.
Elsa Sousa, 05-02-2017 11:14
Re : On Populus sp. bark
I have 2 bad pictures of that feature, one worse than the other. Maybe the excipulum wasn't enough wetted and/or I scratched the slides too much so it broke. However, I think that it could be D. connivens.
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Hans-Otto Baral, 05-02-2017 11:23
Hans-Otto Baral
Re : On Populus sp. bark
It's not clearly seen but I still doubt Durella. D. connivens has an inamyloid hemispherical ascus apex.

The conidia in your last picture belong to a hypho, I suppose Cryptocoryneum.
Elsa Sousa, 05-02-2017 11:54
Re : On Populus sp. bark
Thank you Otto,

Now I have that doubt too. Maybe what I saw isn't a blue reaction, because the second photo already shows a dark zone on ascus apex without iodine, just water.