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Marian Jagers Marian Jagers

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Hypoxylon with angular spores
Steve Clements, 08-08-2016 22:11
Bonjour,
Voici un Hypoxylon (je crois) avec des spores normals ac aussi des spores angulaires.

This effused Pyrenomycete was on a log in a mixed woodland, mostly pine but with some birch and other broadleaf. The purplish-brown surface turned completely black with mostly square-angular spores with a few normal ones after a day, but within 10 days a thick matted crust of normal spores had formed, with an occasional angular spore.
The surface was rather flat, with little sign of perithecial mounds. Perithecia were oval, 0.9 x 0.3 mm. The fungus was about 1.2 mm thick, bounded by a black layer below.
Normal spores were up to 11 x 5, with a long germ slit.
Angular spores were similar in size.
Asci were about 5 -6 wide and up to 150 long, tips blueing in Lugol.
Paraphyses were thread-like.
KOH-extracted pigment was grey-brown rather than orange or red-brown.

Using a key provided by the British Mycological Society:
Stromata widely effused
Stromata with lilac tinge
Stromata not immersed, pigment olive, beige, lilac or absent, so not H. petriniae.
Could this be H. fuscopurpureum?

Cordialement,
Steve
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Jacques Fournier, 09-08-2016 03:35
Jacques Fournier
Re : Hypoxylon with angular spores
Hi Steve,
the rectangular ascospores you observed are just damaged, likely old, and the germ slit is wide open, which accounts for the odd shape. Ascospores still in the ascus feature the typical morphology.
I think you are dealing here with H. macrocarpum Pouzar.
H. fuscopurpureum differs primarily by equilateral spores with perispore indehiscent in KOH and more yellowish pigments.

Cheers,

Jacques
Steve Clements, 09-08-2016 09:44
Re : Hypoxylon with angular spores
Many thganks once again Jacques,
There is always something new to learn!
Steve