Accès membres

Mot de passe perdu? S'inscrire

18-05-2024 10:47

Anna Klos

Goodmorning,We found this tiny ascomycete (max. 1m

25-03-2024 19:14

Juuso Äikäs

I found some small black pyrenos growing on a dead

14-04-2024 20:04

Manak Roman

Hi all,I have two very similar finding last weeken

10-05-2024 17:40

Anna Klos

Good afternoon, Thursday during an inventory we f

17-05-2024 15:17

éric ROMERO éric ROMERO

Bonjour, Un besoin d'aide pour ce Podospora (...?

17-05-2024 16:25

Pavel Jiracek

Erioscyphella lunata, found on a fallen needle of

29-01-2022 21:44

Jan Eckstein

Good evening, apothecia small, yellowish, 150-300

16-05-2024 11:19

Sylvie Le Goff

Bonjour, j'ai récolté sur une branchette de feui

14-05-2024 09:19

Hans-Otto Baral Hans-Otto Baral

Hi, I want to announce for next Sunday 17.00 middl

16-05-2024 11:49

Castillo Joseba Castillo Joseba

De ayer en rama de abetoNo se por donde mirarA ver

« < 1 2 3 4 5 > »
Paraorbiliopsis minuta?
Steve Clements, 12-03-2015 19:10
Hi,
I tried to key out this minute asco using Ellis and Ellis and Fungi of Swizerland and got nowhere. So I resorted to scanning all of Peter Thompson's pictures in Ascomycetes in Colour with spore diagrams which looked right. The only one I was able to match up was Paraorbiliopsis minuta. Because Peter's descriptions are brief, I wasn't over-confident with the ID. The specimen was found on a stick under larch in a mixed wood, so may not be larch – it's an increasingly disturbed woodland right on the edge of Sheffield. The semi-transparent cushion-saped sessile fruit bodies were up to 350 mu (0.35 mm) wide so very small indeed. Colour pale yellow. I wasn't convinced that they were on a subiculum. On microscopy the asci discharged spores – an amazing sight. Hyaline spores with 2 guttules, 9-13.5 x 3-3.5, slightly curved. Asci about 50 x 6-7, tips bluing in Melzer's, inoperculate I think. Paraphyses hard to see but look to be thread-like.
Any comments would be very welcome,
Cheers,
Steve
  • message #34518
  • message #34518
Hans-Otto Baral, 12-03-2015 20:25
Hans-Otto Baral
Re : Paraorbiliopsis minuta?
Dear Steve

P. minuta is excluded because it is inamyloid. What you can do is looking in a squash mount on the margin from exterior (!), so put an apo upside down in the water drop and place the slide over it. Then you can try to look if there are any short hairs at the margin or the mid flanks of the excipulum. I suspect a Hyaloscypha. The genus Parorbiliopsis is undoubtedly nothing but a Hyaloscypha without hairs. Species with amyloid asci generally have tapered hairs, partly rather short and easily overlooked.

Zotto
Steve Clements, 12-03-2015 23:18
Re : Paraorbiliopsis minuta?
Hi Otto, that was very helpful - I did as you said, though it is difficult to manoevre such tiny ascocarps. I think these are the external hairs, mostly cylindrical and blunt rather than pointed, with some even quite capitate. I can't find a good match for any Hyaloscypha in Thompson. I presume that these are quite young ascos and that a fringe of hairs will develop later? I'll keep them in an incubator. I'm beginning to wonder if my microscope is good enough for such tiny structures. These photos are with oil immersion.
(Peter Thompson has Paraorbiliopsis as "blue reaction with iodine" - is this an error? 
Steve
  • message #34522
  • message #34522
  • message #34522
  • message #34522
Chris Yeates, 13-03-2015 02:09
Chris Yeates
Re : Paraorbiliopsis minuta?
Hello Steve
at this level I really wouldn't be looking at Peter Thompson's book  for further  information; the drawings there are, unfortunately, a bit basic; I'll email you some useful stuff re Hyaloscyphaceae - and do look at Zotto's files at https://www.cubbyusercontent.com/pl/CC%2bAscomycota/_1354d48ffaad4b59bd3ffdbb35915d1f#CC%20Ascomycota/7a%20Helotiales     >  Helotiales   > Hyaloscyphaceae without VBs
 
regards
Chris
Hans-Otto Baral, 13-03-2015 08:13
Hans-Otto Baral
Re : Paraorbiliopsis minuta?
Difficult to say, the last image shows excipular cells near the margin, but I cannot detect clear hairs. On the other pics I see living paraphyses without VBs, so something around Calycina/Calycellina seems to be excluded.

No idea.....
Michel Hairaud, 13-03-2015 10:50
Michel Hairaud
Re : Paraorbiliopsis minuta?

Hi Steve, and everyone,


We lately had the very same question on our local SMMA forum a few days ago. A Parorbiliopsis collection was suspected for a tiny disco apparently whithout hairs and negative IKI reaction.


As I had the opportunity to receive and study it living, I discovered unconspicuous hairs not longer than 20 µm long as seen on the attached picture.


I concluded (with the other features, including positive IKI reaction.. in H. albohyalina var. spiralis)


Amitiés


Michel

  • message #34530
Hans-Otto Baral, 13-03-2015 11:05
Hans-Otto Baral
Re : Paraorbiliopsis minuta?
Hi Michel

do you mean your fungus had amyloid asci or not?

If not then such short hairs would point to H. intacta.

Zotto
Steve Clements, 13-03-2015 11:23
Re : Paraorbiliopsis minuta?
Thanks Chris,
That's very kind of you,
Steve
Steve Clements, 13-03-2015 11:26
Re : Paraorbiliopsis minuta?
Hi,
many thanks everyone for the assistance.
I shall record this as Hyaloscyphus sp., which is a new record for the Sheffield wood I am currently surveying,
Steve
Michel Hairaud, 13-03-2015 11:56
Michel Hairaud
Re : Paraorbiliopsis minuta?

Yes Zotto, the first try had showed unamyloid asci , probably due to too old Lugol.


(It was the opportunity to see that n your files the difference is light between H. intacta and the paorbiliopsis sp.)


Michel

Hans-Otto Baral, 13-03-2015 12:57
Hans-Otto Baral
Re : Paraorbiliopsis minuta?
Exactly, they might belong to a single species. IKI-, H-, hairs with or without.