Asci cylindrical, 65-70 x 5-7 microns, aporrinchas, with 8 spores in two rows at the end. I + weak in the end. Paraphyses cylindrical, septate, 2 microns in diameter, containing some slightly greenish, rather dispersed, with no apparent guttae. Spores elliptic-fusiform, 8-9 x 2 microns, with some tiny granules. In the pileus, the external excípulo consists of a thin layer of spherical cells, 8-10 microns, purplish coloration, some of which are small groupings and elements somewhat hyaline pyriform, finely furfuraceous responsible for the appearance that viewed under magnification. In the marginal zone is shaped like a slightly cylindrical elements palisade, hyaline. Inland presents a hyphal weft, with branched hyphae of 2 microns diámetro. La foot structure is somewhat different, for the most part are parallel hyphae with cylindrical cells, many of which are slightly tapered at the ends of 20 - 30 x 4-6 microns, of reddish purple color with slightly thickened walls and many of them presented specific scale, the peripheral cell layer form a shorter but without being spherical, outside, on top there some small and scattered cellular clusters while the lower half are fine hairs hyaline septate, up to 100 x 3 microns.

Ascas cilíndricas, de 65-70 x 5-7 micras, aporrincas, con 8 esporas en dos hileras al final. I+ débil en el extremo. Paráfisis cilíndricas, septadas, de 2 micras de diámetro, algunas con contenido ligeramente verdoso, más bien disperso, sin gútulas aparentes. Esporas elíptico-fusiformes, de 8-9 x 2 micras, con algunos diminutos gránulos. En el píleo, el excípulo externo está formado por una fina capa de células esféricas, de 8-10 micras, de coloración ligeramente violácea, algunas de las cuales forman pequeñas agrupaciones más hialinas y con elementos algo piriformes, responsables del aspecto finamente furfuráceo que se veía a la lupa. En la zona marginal se forma como una empalizada de elementos ligeramente cilíndricos, hialinos. Hacia el interior se presenta una trama hifal, con hifas ramificadas, de 2 micras de diámetro. La estructura del pie es algo diferente; en su mayor parte son hifas paralelas con células cilíndricas, muchas de ellas ligeramente estrechadas en los extremos, de 20-30 x 4-6 micras, de coloración violácea, con las paredes un poco engrosadas y muchas de ellas presentado incrustaciones puntuales; las periféricas forman una capa de células más cortas pero sin llegar a ser esféricas; en el exterior, en la parte superior hay algunas pequeñas y dispersas agrupaciones celulares mientras que en la mitad inferior se presentan finos pelos hialinos septados, de hasta 100 x 3 micras.

Pie. Pileus
Thank you for your interest

Pileus interior

Looks for me a bit like a Sclerotiniaceae. Could you please try to make a photo of an ascus apex in iodine? The ascus should be dead, so perhaps use a flame to shortly boil the mount and then add IKI.
Zotto

Ascus with iodine (lugol)

Well done. Looks quite negative, pff. I think about the genus Moellerodiscus (lentus), but I am not sure.

M. lentus has a few regular oil drops in the spores. In your specimen this is not clear to me.
Zotto

I watched your DVD on Moellerodiscus and certainly has a similarity, likewise another specimen on Ceratonia siliqua, also is very similar. It is curious that harm the soil where it is, Eucalyptus camaldulensis, not much information?. I can only send this picture of spores than what has already sent.
Thanks, as always.
Kind regards
Javier

yes, on this spore pic 2-3 spores look fine with their oil drops. Most if not all are dead, therefore the oil drops are not regular.

Your statement of aporhynchous ascus bases is very important. Are you sure? M. lentus has usually croziers, but we have one collection (by Michel Hairaud on old apples) which is actually aporhynchic.

It is best to mount in KOH + Congo Red to see the croziers clearly.

Zotto

Hi Javier and Zotto
I cannot talk about Javiers find on bark Eucalyptus. Maybe Javier can let me study one sample?
But we are used to collect here at El Saler a similar species on Ceratonia siliqua. It mostly grows on ¿stromatized? seeds that are still inside the pod, but also on pods, and rarely on little twigs and petioles. I think that it is very similar to the find of Michel Hairaud on Malus fruits (HB 6724). In our case also there are no croziers, but in some asci you can see an incomplete crozier or basal protuberance (you have to seek a lot and it not appears in all apothecia). Moreover, the cells of the ectal excipulum are inconstantly amyloid in IKI and amyloid in IKI after KOH pre-treatment.
The same species but so different host? Very difficult group...
I attach more pics of the species that grows on Ceratonia siliqua in the pdf file.

No puedo hablar de la recolecta de Javier en corteza de Eucalyptus. ¿Me guardas una muestra Javier? Pero estamos acostumbrados a recolectar aquí en El Saler sobre Ceratonia siliqua una especie parecida. Crece sobre todo en semillas ¿estromatizadas? que están todavía dentro de la vaina, pero también en las vainas y más raramente sobre pequeñas ramitas y peciolos. Creo que es muy similar a la recolecta de Michel Hairaud sobre manzanas (HB 6724). En nuestro caso tampoco hay uncínulos basales pero en alguna asca se puede ver un uncínulo incompleto o protuberancia basal (hay que buscar mucho y hay apotecios en los que no aparecen). Además las células del excípulo ectal son inconstantemente amiloides en IKI y amiloides en IKI tras un pretratamiento con KOH.
¿La misma especie en huéspedes tan diferentes? Es un grupo muy difícil...
Adjunto más imágenes de la especie sobre Ceratonia siliqua en el pdf.

Raúl

Hi Raul

it is strange but I have only some scans of diapositives about these Ceratonia finds. Are they yours? I have no info about that, and not on the ascus base either.

Could you please send me the photos in the attached pdf by mail, at best as single jpg files?
thanks

Zotto

No, not mine. I send the pics now to your e-mail.
Raúl