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Hepaticolous, tiny, and enigmatic
Michel Hairaud, 21-01-2015 14:49
Michel HairaudHi to every one,

I am looking for help after the collection of a tiny, stipitate, white- hyaline creature on (I think ?) Frullania. on Picea standing and living trunk.


Apos from 0,1 to 0,2 mm .


Ascus 50 - 80 x 11-12,5 , IKI negative , H+, clavate with a narrow base


Paraphyses with the last cell very broader , with CRB + vacuolary content


Spores with OCI 4,5 , most often with 2 larger guttules x 2,8 µm and smaller ones, 13-15 x 3-3,5 µm , mostly 1x septate oustise ascus (and also sometimes inside) .


I apologize for the rather poor picture quality and the lack of information on the excipulum (at least)


Amitiés et merci, Michel

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Hans-Otto Baral, 21-01-2015 15:15
Hans-Otto Baral
Re : Hepaticolous, tiny, and enigmatic
Hi Michel
the pictures look wonderful! I have a slight idea: Phialina anomala.  I have this in my Hamatocanthoscypha folder, despite of the strong VBs. That fungus is sessile, however, and has short +/- curved hairs.

Your fungus has VBs as I can see in the IKI photo. If you have still material, please do a photo showing VBs in water, and perhaps also an external view on the excipulu, without pressure (possible hairs, VBs).
Zotto
Michel Hairaud, 22-01-2015 10:27
Michel Hairaud
Re : Hepaticolous, tiny, and enigmatic

Merci Zotto,


You can see VBs (weekly refractive and rather homogeneous)  in water in image 3 above and I join more pics but I still have difficulties to understand the fungus structure . No curved hairs anyway.


I would have excluded a Hyaloscyphaceae


 


Amitiés Michel


 

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Hans-Otto Baral, 22-01-2015 12:12
Hans-Otto Baral
Re : Hepaticolous, tiny, and enigmatic
The Hyaloscyphaceae you must revise in your mind, take only Parorbiliopsis minuta and Hyaloscypha intacta, they are perhaps even the same species.
Michel Hairaud, 23-01-2015 21:48
Michel Hairaud
Re : Hepaticolous, tiny, and enigmatic

Hi again,


I wonder whether these new images could help :


- the outer surface develops as an anamorphic state (the same process I observed in about twenty ''apos''), the swollen terminal cells react in IKI as if containing VBs . These cells appear on the surface and among asci


- With KOH before IKI, the ascus walls totally becomes blue


Michel


 

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Hans-Otto Baral, 23-01-2015 22:25
Hans-Otto Baral
Re : Hepaticolous, tiny, and enigmatic
What a surprise! such iodine reaction is very rare in the Hyaloscyphaceae (Venturiocistella I remember). I think now only a sequence could help :-)

But this habitat should have been searched intensely, by JPP for instance....

Could you clarify the host genus?
Enrique Rubio, 14-03-2022 19:13
Enrique Rubio
Re : Hepaticolous, tiny, and enigmatic
We have also found this fungus, and I wonder if you have finally come to any conclusions.
Many thanks in advance
Michel Hairaud, 14-03-2022 20:56
Michel Hairaud
Re : Hepaticolous, tiny, and enigmatic
Bonsoir Enrique, 
Nice that you did find it !
There was unfortunately no conclusion for this find. 
Among other mycologists consulted, Peter DObbler suggested the taxon Vezdaea , here is his answer :
should be compared with the lichen genus Vezdaea, e. g. V. stipitata. It is presumably accidentally on withering hepatics (surely Radula complanata) where algae are present. The iodine reaction is typical of Lecanorales. Good luck! Peter


AmitiésMichel
Hans-Otto Baral, 14-03-2022 21:21
Hans-Otto Baral
Re : Hepaticolous, tiny, and enigmatic
Finally it is clear! Vezdaea stipitata. In Lecanorales, Vezdaeaceae.

Pierre-Yves Courrio had it, vid. JPP.
Enrique Rubio, 15-03-2022 10:19
Enrique Rubio
Re : Hepaticolous, tiny, and enigmatic
Thanks a lot Michel and Zotto
Reallys a nice fungus!
Here are my pics.
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Hans-Otto Baral, 15-03-2022 10:23
Hans-Otto Baral
Re : Hepaticolous, tiny, and enigmatic
And especially we must thank Peter Döbbeler for the hint.
Enrique Rubio, 15-03-2022 10:28
Enrique Rubio
Re : Hepaticolous, tiny, and enigmatic
Of course.
Michel Hairaud, 15-03-2022 10:38
Michel Hairaud
Re : Hepaticolous, tiny, and enigmatic
Very nice pics , ENrique, thanks for sharing. 
I wonder how you came across my post on Ascofrance from 2015 as there was then no information on a possible lichen sp. ? May be from the subject containing hepaticolous ? 

Amitiés. Michel
Enrique Rubio, 15-03-2022 10:42
Enrique Rubio
Re : Hepaticolous, tiny, and enigmatic
I understand your surprise, Michel. I was not sure if apothecia grew on Frullania and thanks to the search for this word I found the solution to this problem.
Long live Ascofrance!
Michel Hairaud, 15-03-2022 10:53
Michel Hairaud
Re : Hepaticolous, tiny, and enigmatic
Oui, formidable Ascofrance ! Et formidables mycologues actifs sur ce site !
Nous sommes heureux de contribuer à la poursuite de son fonctionnement . 
Michel