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09-01-2023 08:14

Francisco  SÃNCHEZ Francisco SÁNCHEZ

Ascomiceto anaranjado sobre suelo desnudo, 29/12/2

27-05-2024 22:10

Ethan Crenson

Hi everyone, I found these lovely stipitate ascos

26-05-2024 12:05

Alain Delannoy

Bonjour,Je n'arrive pas à mettre un nom sur cette

25-05-2024 16:40

Sylvie Le Goff

Bonjourj'aimerais confirmation ou pas pour ce peti

25-05-2024 18:29

Malcolm  Greaves Malcolm Greaves

I found a group of Mitrula gracilis? on wet debris

25-05-2024 16:54

Sylvie Le Goff

Bonjour j'aimerais confirmation ou infirmation de

22-05-2024 23:39

Marc Detollenaere Marc Detollenaere

Dear Forum,On debarked Fagus I found some small wh

21-05-2024 17:48

Karl Soler Kinnerbäck

Hi all,Could this be Venturioscypha or Venturiocis

21-05-2024 11:33

Nihad Omerovic

Hello,found on dead, dry, attached (and fallen) tw

07-11-2018 08:34

Zuzana Sochorová (Egertová) Zuzana Sochorová (Egertová)

Hello, could someone send this publication to me

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Cistella on dicot
B Shelbourne, 13-04-2024 21:10
B Shelbourne• Genus Cistella on dicots: Habitat, macro, hairs, and spores.
• Possibly C. rubrotincta (from Zotto's folders): Spore size, long and tapering hairs.


Habitat: On dead, hollow, standing stems of herbaceous plant, 2-3 cm wide, lower part of stem but not base, damp when found, not seen on several other stems nearby, in a clearing, no bodies of water in the immediate vicinity, mixed deciduous woodland, mostly Betula pendula and Quercus robur, Low Weald, England (south), mid-April, after rain.


Associates: Lots of small brownish conidiophores, and a few larger black apothecia – possibly Pyrenopeziza (seems to be a common associate).


Apothecia: 20+ on the stem, in a loose group, generally scattered but occasionally 2-caespitose, 0.2-0.3 (0.5) mm diameter without hairs (< 0.6 mm with hairs), < 0.1 mm high (1.5 mm with hairs), pale yellowish with a hint of pink (apricot), shallow cupulate to discoid, translucent, gelatinous appearance and texture; margin distinct, more in-rolled until maturity, like receptacle; hairs whitish, more abundant and longer at the margin, when immature thick and white, when mature appearing conglutinated in regular groups, and pointing outwards; disk more translucent, greyish due to dark substrate, gelatinous appearance, eventually convex.


Storage and method: A short piece of stem stored overnight in a damp box, sections taken from a single large mature-looking apothecium, mounted in tap water (unfortunately median section was a little thick and turned hymenium down), a little pressure applied to separate cells of the hymenium, IKI added to water mount, KOH + CR added after IKI.


IKI: Apical rings weakly bb, some dextrinoid ascoplasm, hairs with yellow contents.
KOH + CR: Hairs strongly red, gel at apices dissolving, ornamentation remaining; exudate more greenish.


Asci: Cylindrical to mildly clavate, apex conical, IKI bb, croziers, 1-2-seriate, many discharging in water mount,.
• Living mature: ~ 33-45 x 4.5-5 (5.7) um, pars sporifera ~30-40%, apex more rounded-conical, spores diagonally oriented, one spore at apex, sometimes with hyaline to greenish VBs above base of ascus.
• Dead mature: ~30-38 x 3.5-4.5 um, pars sporifera ~60-90%, apex more acute-conical, small apical thickening ~1 um, spores more vertically oriented
• Discharged: Small poricidal opening, cylindrical, apex acute, usually with some amyloid material remaining at the extreme apex but once without, still relatively turgid, many small LBs.


Spores: Narrow-ellipsoid to fusiform, poles rounded to acute, usually heteropolar with the base more attenuated and acute (apex more rounded-obtuse), a few tiny shadowy LBs around the poles, OCI 1.
Vital spores measured in water mount: (6.9) 7.1-8.0 (8.7) × 1.7-2.1 µm, Q = (3.5) 3.6 - 4.2, n = 11, mean = 7.5 × 1.9 µm, Q mean = 3.9.


Paraphyses: Gradually lanceolate to filiform, 2.5-3 um wide, apex rounded-acute, ~3 septa, exceeding immature asci, very faint yellowish VBs (very lowly refractive and more noticeable at lower magnification), several shadowy LBs more noticeable in KOH + CR.


Marginal hairs: ~40-60 x 5-6 um, apices ~2.5-3 um wide, abundant at margin, some at flank, broad-cylindrical, tapering, sinuous, apex rounded, protruding part observed with 3-4 septa, 1-2 apical cells with very light ornamentation of tiny warts, ornamentation remaining in KOH, longer hairs with tangled apices, apparently conglutinated, bases apparently with VBs like paraphyses; some shorter hairs, 30-40 um long, clavate apical cell, apparently unornamented.


Exudate: Yellow-brown, thick, globular, gelatinous, on surface of ectal excipulum and short hairs around base.


Ectal ex: Very light yellowish at low magnification, hyaline at high magnification, surface with textura prismatica, internally possibly textura globosa.

  • message #79021
  • message #79021
  • message #79021
  • message #79021
  • message #79021
B Shelbourne, 15-04-2024 13:30
B Shelbourne
Re : Cistella on dicot
I think the stem could be Apiaceae, so with the spore size I'm wondering about C. grevillei.
Michel Hairaud, 15-04-2024 20:07
Michel Hairaud
Re : Cistella on dicot
Hi , that's also my guess 
Amitiés
Michel
B Shelbourne, 16-04-2024 10:45
B Shelbourne
Re : Cistella on dicot
Thank you for looking.

This is my first Cistella sp., and some of the smaller spored species on dicots look quite similar. I didn't find any accessible key and I'm not sure about the substrate preferences, but I looked through Zotto's folder.

I did see the paper of Quijada et al. (2015) on Hyphodiscus and Cistella in the Canary Islands. Based on their suggestions (following Raitviir), the length of the spores and asci seem to fit better with C. hungarica, but I didn't see this species in Zotto's folder.

B Shelbourne, 16-04-2024 10:54
B Shelbourne
Re : Cistella on dicot
I see now that these are synonymised in Zotto's folder. That is good to see because the spore sizes reported in Zotto's folder are quite variable, and some of those I measured are a little too long compared to observations of Quijada et al.