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24-07-2024 14:07

Elisabeth Stöckli

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Thomas Flammer

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Hans-Otto Baral Hans-Otto Baral

Hello please note that I uploaded today an Englis

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Kozak Robert

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Masanori Kutsuna

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Villalonga Paco

Small Scutellinia growing in garden soil (calcareo

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Claussenomyces? on twig of Abies
Robin Isaksson, 28-01-2024 19:20
Robin IsakssonHi

 Found this one on a twig of Abies in Sweden.

Apothecia paler when wet.
Apothecia react K+ yellow floating substance. 

Asci 8, 100-130 x 12-14, IKI -
Spores at fist aseptate finaly 1 septate, 16-19 x 8-9.5 
paraphyses a litle wider at top, septate

Anyone knows it?

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Hans-Otto Baral, 28-01-2024 21:42
Hans-Otto Baral
Re : Claussenomyces? on twig of Abies
Although the asci are narrower and the spores uniseriate, this sounds to me like Patinella punctiformis. I have it actually in Tympanidaceae, folder Pragmopora-like.

This is among my wishes for a sequence, I studied the type but never saw a further collection, except for one on Quercus.

Could you please measure the apo diameter? I am not sure with my 0.3-0.7 mm. Rehm measured 0.2-0.4 mm.
Robin Isaksson, 28-01-2024 21:57
Robin Isaksson
Re : Claussenomyces? on twig of Abies
Dear Zotto,

Amazing as usual!

Its a good collection with many apothecia.
Regarding sequnce it i will try to convince my friend to do it.    

I will measure some apothecia and take pictures tomorrow. 

All the best,


Robin
Adam Polhorský, 28-01-2024 22:36
Re : Claussenomyces? on twig of Abies
Hello, this should be Pseudotryblidium neesii. Not that rare on Abies bark. Patinella punctiformis f. quercina is probably related indeed, I've seen it 2 times on Quercus bark. Not sure about Patinella punctiformis.

Adam
Hans-Otto Baral, 29-01-2024 09:49
Hans-Otto Baral
Re : Claussenomyces? on twig of Abies
Good hint, Adam! I overlooked the KOH-reaction mentioned by Robin. That species I have in Dermateceae.

For the type of Patinella punctiformis on Picea bark I already had the note: "Similar to Pseudotryblidium neesii, but without ionomidotic reaction".

For P. neesii exist sequences in GenBank, so no need for this one.
Robin Isaksson, 29-01-2024 21:44
Robin Isaksson
Re : Claussenomyces? on twig of Abies
Dear Adam and Zotto,

Thank you!

I did cut some moore apothecia, after some time i did found 2-3 septate spores in asci.
The asci is weak react brownish red in K.

I can not se the red/ purpurle reaktion in K.
In my it become a brown floting lequid.

Maybe this is okey? as it is a new species to Sweden.

Adde pictures seen in KoH 10%
Best regards
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Hans-Otto Baral, 30-01-2024 20:32
Hans-Otto Baral
Re : Claussenomyces? on twig of Abies
This is distinctly a ionomidotic reaction. The asci and spores are in KOH +  iodine?  I see no reaction of either of them.
Robin Isaksson, 30-01-2024 22:03
Robin Isaksson
Re : Claussenomyces? on twig of Abies
All picture Above in already sent is only water+ koh

Here is 2 pictures of asci in water +koh.
Reaction best seen in asci without spores and not all get this reaction.


Last picture is koh + lugols
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Hans-Otto Baral, 31-01-2024 11:14
Hans-Otto Baral
Re : Claussenomyces? on twig of Abies
Ah, you mean the ascoplasma (and sporoplasma) reacts in a similar colour as the pigment extruding from the excipulum. That is right, although hyaline when alive such coloured plasma often occurs in dead cells, I am not sure if KOH is necessary for that.