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Thomas Flammerdark brown apothecia, splitIKI-Spores biguttulate

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Thomas Læssøehttps://svampe.databasen.org/observations/10527903
Orange, dark-stipitate apothecia on alga-covered wood in flood zone
Edvin Johannesen,
09-02-2023 16:29

I am still trying to figure out vital taxonomy/microscopy. The images below are from material which has been dry for months, mounted in water (except for the IKI one, in Lugol). I hypothesize that we see living spores inside (and outside) dead asci, but I'm not sure. The spores measure approx. 5-6 x 15-17.5 micr. Asci 90-100 x 7.5-10 micr. Spores contain a few large and sereval small guttules. Paraphyses are a bit difficult to interpret clearly - some seem filiform, some thicker and acuminate, some brownish with granular content. I assume they are dead? I have not seen croziers. The ascus pore stains blue in Lugol (no pre-treatment)
I have been thinking Phaeohelotium, but I am not sure how that genus is cureently to be understood. I don't see a distinct layer with rounded cells in the excipulum.
Any suggestions are welcome. Thanks.
Edvin Johannesen,
09-02-2023 16:30
Edvin Johannesen,
09-02-2023 16:33

Re : Orange, dark-stipitate apothecia on alga-covered wood in flood zone
Forgot to mention that the spores eventually become 1-septate, as you can see in one of the images.
Hans-Otto Baral,
09-02-2023 17:13

Re : Orange, dark-stipitate apothecia on alga-covered wood in flood zone
To me this looks a bit senescent. I more think of a Hymenoscyphus, H. calyculus perhaps. That should have croziers, however.
Edvin Johannesen,
09-02-2023 23:03

Re : Orange, dark-stipitate apothecia on alga-covered wood in flood zone
OK, I will check. Thanks a lot.