Accès membres

Mot de passe perdu? S'inscrire

20-07-2022 20:37

Elisabeth Stöckli

Bonsoir, Sur bois mort de Pinus cembra en situati

23-07-2022 12:51

Mirek Gryc

Hi.Looking for a name for this species would take

22-07-2022 20:21

Malcolm  Greaves Malcolm Greaves

Has anyone a copy of the Mycol. Progr. 14(6): 41

20-07-2022 18:35

Mirek Gryc

HiDo you have any ideas?Ascospores: Me = 6.7 × 3.

18-06-2022 14:10

Elisabeth Stöckli

Bonjour, Sur branche morte de Pinus cembra en pos

18-06-2022 00:01

Viktorie Halasu Viktorie Halasu

Hello forum,could someone send me a copy of this p

17-07-2022 23:10

Przemyslaw Drzewiecki

July 16, 2022, Poland - Mochle; Pasture for horses

01-07-2022 15:18

Thomas Læssøe

https://svampe.databasen.org/observations/10278120

16-07-2022 15:17

Juuso Äikäs

I found a few Lachnum fruitbodies growing on a pie

16-07-2022 08:58

Castillo Joseba Castillo Joseba

De ayer en madera degradada de fagusA ver si hay a

« < 210 211 212 213 214 > »
White microfungus (airborne contaminant ?) with swellings along its hyphae
Zetti Mario, 16-07-2022 17:01
Hi, I am doing some work with microfungi and during isolation of some Eryphise I have got a bonus colony (isolated in pure form now) which is cotony white and raised by some 4mm from the medium's surface. Could be an airborne contaminant, but maybe it was on the host plant. The colony had a slight scent (faint aromatic maybe...)

Under the microscope, I cannot observe free conidiospores but the hyphae were regularly swollen into thick-walled vesicles (zoospores?). Any suggestions are welcomed. Reminds me in the distance to Phythphtora...
  • message #73387
  • message #73387
  • message #73387
  • message #73387
  • message #73387
David Malloch, 16-07-2022 20:35
David Malloch
Re : White microfungus (airborne contaminant ?) with swellings along its hyphae
That will be difficult to identify. The hyphae are producing thick-walled intercalary cells and chlamydospores, but nothing else. It could be a Fusarium, but many other things as well. When I encounter fungi like that, I try a different medium. Maybe V8 or something with a lot of cellulose but not much sugar. Good luck!
Zetti Mario, 17-07-2022 08:07
Re : White microfungus (airborne contaminant ?) with swellings along its hyphae
Thank you, interesting. I was working with a Fusarium oxysporum recently. I have available Sabouroud agar, CZapeK DoX, PDA, Oat Agar and MEA. Which medium do you suggest (Oat perhaps?) - TNX
David Malloch, 17-07-2022 13:45
David Malloch
Re : White microfungus (airborne contaminant ?) with swellings along its hyphae
Those media mostly have a high C/N ratio that may encourage vegetative growth at the expense of sporulation. Perhaps the oat agar would be the best choice.
Zetti Mario, 18-07-2022 08:47
Re : White microfungus (airborne contaminant ?) with swellings along its hyphae
Thank you, Oat Meal and PDA innoculated and I will report back the results.
Zetti Mario, 08-08-2022 18:32
Re : White microfungus (airborne contaminant ?) with swellings along its hyphae
Hello David and everyone.

I managed to find time to reply you back. I managed to get a pure colony on PDA and Oat indeed it was a Fusarium, probably F. oxysporum s.l. (maybe lycopersici) I followed a simple key so dont take this as final determination of course!

I was intrigued by pinkish-peach coloration (images below has enhanced colour)  in the 12 day old colony. They were like soft non encapsulated tissue. Under the microscope they looked like bunch of elongated grapes. Is this characteristic of the species ? Are they the sporodochia ? 


I saw some papers showing this pinkish-peach coloration by F. oxysporum s.l. - e.g.  https://www.frontiersin.org/files/Articles/335707/fmicb-09-00420-HTML/image_m/fmicb-09-00420-g001.jpg

from https://www.frontiersin.org/articles/10.3389/fmicb.2018.00420/full

and this is a good reference paper too
https://repository.naturalis.nl/document/668094
  • message #73649
  • message #73649
  • message #73649
  • message #73649
  • message #73649