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12-06-2026 14:50

François Freléchoux François Freléchoux

Bonjour, Voici la brève description d'une Mollis

10-06-2026 21:16

François Freléchoux François Freléchoux

Bonsoir,Le dernier du jour, en attendant votre avi

11-06-2026 16:24

Ethan Crenson

Hi all, Recently, a friend found this Orbilia in

11-06-2026 19:01

William Slosse William Slosse

Hello all,In an attempt to make a culture of a sus

11-06-2026 19:03

Nicolas VAN VOOREN Nicolas VAN VOOREN

Chers membres d'Ascofrance,Le site sera placé en

10-06-2026 23:08

éric ROMERO éric ROMERO

Bonjour tous, Je vous propose un Mollisia trouvé

09-06-2026 18:32

Camille Mertens

Sur morceau de roseau immergé 0,5 - 0,7 mm de dia

10-06-2026 12:54

Steve Clements

Bonjour encore, Pouvez-vous m'aider, s'il vous pl

10-06-2026 21:07

François Freléchoux François Freléchoux

Toutes les tiges de gentianes jaunes de l'an passÃ

10-06-2026 13:41

François Freléchoux François Freléchoux

Bonjour à nouveau, Voici une trouvaille d'hier.

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Is it Gliocladium ???
Stephen Martin Mifsud, 23-10-2020 12:02
Stephen Martin Mifsud

Hi, I found small white-cream colonies on decaying seeds of Washingtonia, which I cultured. The resulting colonies where fluffy ash-white with a widespread maube-violet central zone. Under the microscope, the first thing that was stricking was the irregular size of the spores, ranging from 5 to 10um long. Spore dimensions from pixmetere:
(5.7) 6.4 - 9.6 (10.4) × (3.3) 3.4 - 4.9 (5.9) µm
Q = (1.5) 1.6 - 2.2 (2.7) ; N = 27
Me = 8 × 4.2 µm ; Qe = 1.9


The spores were terminal, solitary on undifferentiated hyphae, similar to the mycelium, but sometimes they appeared to be in small clusters, and occassionally I saw a penicillate arrangement on very long conidiophore.


I dont know if this is some irregular or not well known Gliocladium sp.

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David Malloch, 23-10-2020 19:59
David Malloch
Re : Is it Gliocladium ???
Hello Stephen,

Perhaps this is a member of the Microascaceae. The habitat on decomposing seeds would be common for that family.  The conidiophores and hyphae remind me of Scedosporium or perhaps an early stage of a Graphium. Watch for ascomata of Petriella or Pseudallescheria in your cultures.

David
Stephen Martin Mifsud, 23-10-2020 21:14
Stephen Martin Mifsud
Re : Is it Gliocladium ???
Thank you David, I will continue my investigatoion in that direction you kindly suggested and I think you are correct. There are many things which are matching, habitat, habit, variety of conidiophores (short, long, synnematous), spores with truncate bases and if I understood the annelid conidiophores with reference to wrinkled sides (seen in one of my images without congo red). The cottony colony with a dark centre is also diagnostic.

This genus is rather complex forming different reproductive structures - myself I was confused if I have a contamination of two species.

I examined my cultures by taking a film of mycelia from the surface with cellophane tape. I will investigate further down. Pure subcultures are growing fine too. Seems to be a hard one re determination to species! 

Thank you for replying and sharing your knowledege!


Important references

http://website.nbm-mnb.ca/mycologywebpages/Moulds/Scedosporium.html
http://thunderhouse4-yuri.blogspot.com/2012/10/scedosporium-prolificans.html