Spores: 1-septate

(9.3) 9.6 – 12.7 (15) × (2.8) 3.2 – 4 (4.4) µm
Q = (2.5) 2.6 – 3.6 (4.7) ; N = 35
Me = 11.1 × 3.6 µm ; Qe = 3.1

12.19 3.37
9.86 3.75
10.87 4.08
13.47 3.33
9.76 3.19
9.54 3.84
9.57 3.58
9.58 3.32
11.01 3.56
10.52 3.48
14.48 3.06
11.49 3.64
9.72 3.10
9.47 3.38
10.68 3.34
11.03 3.34
14.67 4.10
10.79 3.86
12.39 3.99
12.25 3.66
10.68 3.63
10.09 3.46
9.92 3.49
12.35 4.05
12.66 3.76
15.04 4.39
9.69 3.44
9.72 3.80
10.49 3.58
10.62 4.04
10.62 3.60
9.99 3.35
12.07 2.83
9.32 3.70
10.89 3.16

Substrate: overmature polypore

Habitat: mixed woods (maple, birch, hemlock, pine, cedar)

Ecoregion: Eastern Forest Boreal-Transition (NA0406)

Collectors: D. Newman

Collected during the 2017 Peck Foray held at the SUNY ESF Newcomb Campus in Newcomb, New York.

also seen at:

https://www.facebook.com/groups/ascomycetes/permalink/1895848247334037/
http://mushroomobserver.org/293285


NOTE: I cannot shrink my images down to 150KB to be uploaded here. they may be viewed at either of the two above links.

Hans-Otto Baral, 05-10-2017 06:51

Re : "Discomycetes" - 293285

Sorry but in MLZ I cannot identify fungi. 

Looks for me like Calycina (Bisporella) citrina which may occasionally go on polypores. 
But asci should show a reaction, even in MLZ (clearer the reaction would perhaps be in IKI).

Is the excipulum gelatinised and cartilaginous?

Jason Karakehian, 05-10-2017 17:33

Re : "Discomycetes" - 293285

I have collected this a few times now - Maine, Massachusetts and New Brunswick. I determined it to B. citrina. These collections were on old, rotten Dadaleopsis confragosa polypores and on the surrounding wood. Korf believed that B. citrina was a parasite of this. See Korf and Carpenter, Mycotaxon 1(1) p. 57. 1974. I attach a few photos from different collections here.

• 
• 

Hans-Otto Baral, 05-10-2017 18:39

Re : "Discomycetes" - 293285

This is a problematic case.

First: The type of Bisporella, B. pallescens (Helotiaceae), is not parasitic on Bispora, but Bispora is, unlike Fuckel and Korf & Carpenter assumed, the anamorph (we proved that in culture but did no publish the result except for the mere citation of this relationship in Syllabus 2016. 

Second: Bisporella is heterogeneous, a majority belonging to Calycina (Pezizellaceae), including citrina and sulfurina/claroflava. These two species are indeed facultatively fungicolous. For instance, the type of Parthenope parasitica Velen., which is a synonym of C. citrina, grew on a polypore.

Zotto

Danny Newman, 05-10-2017 18:45

Re : "Discomycetes" - 293285

reading this:

http://www.gbif-mycology.de/HostedSites/Baral/IodineReaction.htm
https://invivoveritas.de/articles/iodine-reaction-in-ascomycetes-why-is-lugols-solution-superior-to-melzers-reagent/

and realizing the err of my ways. Is Baral's Solution preferable to Lugol's? How is it made/where can it be purchased?

Many thanks for your conitnued assistance.  Will look at the excipulum next.

-Danny

Hans-Otto Baral, 05-10-2017 19:01

Re : "Discomycetes" - 293285

Thanks for looking at my homepage. Well, European reagent sellers have it, and it is applied here by many. Some even ascribe it to me (in the higher concentration), but it was a French physician who introduced it.

The antiseptic Lugol I was thinking is in alcohol, which is not suitable for the present purpose. Lugol has usually a weak concentration (0.3%), but when I look here
http://en.academic.ru/dic.nsf/enwiki/751372/Lugol%27s_iodine
I see a concentration over 5%!

The solution should be deep brown, minimum 0.5%, not looking yellow in a glass bottle. Either you buy crystalline iodine and KI and mixed it in tap water, or you buy the solution.
Zottto

Danny Newman, 05-10-2017 19:08

Re : "Discomycetes" - 293285

would this be sufficient?

https://www.amazon.com/J-CROWS%C2%AE-Lugols-Solution-Iodine-2/dp/B001AEFM9Y

Hans-Otto Baral, 05-10-2017 19:25

Re : "Discomycetes" - 293285

It actually seems so! The answer on the question of the dosage on bottle was:

each drop contains 1 mg iodine and 1.5 mg potassium iodide,

which is actually exactly the formula for Lugol.

1g iodine in 1 l water would be 1%, the optimum concentration.

1000 drops of this Lugol are certainly not 1 l, so the concentration must be very high in this reagent. But you can easily dilute it.

Good is to keep a high concentrated solution behind because iodine escapes slowly from the solution via plastic lids.

Danny Newman, 05-10-2017 20:06

Re : "Discomycetes" - 293285

i'm not sure I understand.  the formula is the exact same as the ideal Lugol's used in mycological applications, but it still needs to be diluted?  if so, how would it best be diluted?  one drop distilled water, one drop Lugol's?

Hans-Otto Baral, 05-10-2017 21:10

Re : "Discomycetes" - 293285

The relation between I2 and KI is exactly the same as in the current Lugol (1 : 3), but the concentration seems to be so high that it probably should be diluted by adding maybe 5-10 parts of water to 1 part of the solution. Of course I would dilute a small quantity only.