27-11-2025 12:01
Thomas Læssøehttps://svampe.databasen.org/observations/10496727
27-11-2025 11:46
Thomas Læssøehttps://svampe.databasen.org/observations/10493918
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Heather MerryleesHi there!I am hoping for any advice on the identif
29-11-2025 08:40
Andreas Millinger
Hello,on a splintered part of a branch on the grou
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Nogueira HéctorNovember 23, 2025 Requejo de Sanabria (León) SPAI
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Thomas Læssøehttps://svampe.databasen.org/observations/10490522
27-11-2025 15:41
Thomas LæssøeSpores brownish, typically 4-celled; 26.8 x 2.4;
27-11-2025 11:31
Thomas LæssøeCollectors notes: Immersed ascomata, erumpent thro
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Thomas Læssøehttps://svampe.databasen.org/observations/10534623
26-11-2025 18:13
Jason Karakehian
The entire run of Mycotaxon is now available throu
Demande
René Dougoud,
27-05-2015 09:48
J'ai trouvé dans Mycologia 71, 1979, un article de R.J. Bandoni: Safranin O as a rapid nuclear stain for fungi. (chez Cyberliber)
Ma demande : Avez-vous de l'expérience avec ce colorant nucléaire ?
Merci de vos réponses
René
Carmel Sammut,
28-05-2015 18:41
Re : Demande
Hi René,
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
