23-01-2015 16:06
Malcolm Greaves
This second Ascobolus was found on Alpaca dung but
23-01-2015 15:59
Malcolm Greaves
This was on gypsy horse dung. As the gypsys and th
22-01-2015 17:40
Vasileios Kaounas
Found 22-01-15, in sandy soil, in forest with Pinu
23-01-2015 01:21
Miguel Ángel Ribes
Good night againThis yellow-pink Orbilia was growi
22-01-2015 12:32
Vasileios Kaounas
Found 22-01-15, in Greece, in forest with Pinus ha
21-01-2015 00:37
Nick AplinSalut à tous, I found this inconspicous Orbili
Demande
René Dougoud,
27-05-2015 09:48
J'ai trouvé dans Mycologia 71, 1979, un article de R.J. Bandoni: Safranin O as a rapid nuclear stain for fungi. (chez Cyberliber)
Ma demande : Avez-vous de l'expérience avec ce colorant nucléaire ?
Merci de vos réponses
René
Carmel Sammut,
28-05-2015 18:41
Re : Demande
Hi René,
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
