02-06-2015 05:57
Maren Kamke
Hi everybody,apothecia beneath the surface and bre
02-06-2015 11:24
Castillo Joseba
El pasado mes de marzo me enviaron Muestras of thi
06-05-2015 18:02
Bernard CLESSE
Bonjour à tous,Voici ce que je pense être un Lac
01-06-2015 20:33
Enrique Rubio
Hi to everybody and specially to J. Fournier, a ve
31-05-2015 18:02
Bernard CLESSE
Bonjour à tous,J'ai photographié il y a un mois
31-05-2015 19:08
Bernard CLESSE
Je pense bien le reconnaître maintenant et j'essa
01-06-2015 16:02
Chris Yeates
Bonjour touswhile preparing some images of Lachnum
Demande
René Dougoud,
27-05-2015 09:48
J'ai trouvé dans Mycologia 71, 1979, un article de R.J. Bandoni: Safranin O as a rapid nuclear stain for fungi. (chez Cyberliber)
Ma demande : Avez-vous de l'expérience avec ce colorant nucléaire ?
Merci de vos réponses
René
Carmel Sammut,
28-05-2015 18:41
Re : Demande
Hi René,
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
