Bonjour !

Voici un Orbilia probable de la région de Québec.
Merci de toujours nous aider dans nos identifications.

Amitiés !  Roland

Données :      

Date de récolte : 10 / 07 / 2-12
Substrat: feuillu pourri probable

Hyménium couvert d'une substance gélatineuse recouvrant les paraphyses et asques d'une coiffe foncée (épithécium ?)
Asques à 8 spores bisériées, cylindriques, atténués à la base, non amyloïdes ?,18,5-27 x 2,5-4 µm
Paraphyses cylindriques, 1,9 à 2,6 µm de diam.
Spores courtement filiformes à cylindriques, parfois légèrement arquées, hyalines, avec deux petites guttules polaires, 4,8-7 x 0,6-1 µm, 5,9 x 0,8 µm en moyenne, Q = 5,1=10, Q moyen =  7,6
Medulla ± celluleuse, formée de cellules subglobuleuses à cylindriques
Excipulum (ental ?) en textura porrecta, formé d'hyphes ventrues, à paroi épaissie jusqu'à 2,8 µm, septées, 23,5-68,5 x 13,5-33,5 µm
Excipulum ectal (latéral ?) en textura prismatica, formé d'hyphes septées, à paroi épaissie, courtes, 3,5-14 x 2-6 µm
Subiculum formé d'hyphes peu abondantes, subglobuleuses à la base, septées, à paroi épaissie jusqu'à 1,4 µm, 4,2-10,2 µm de diam.

Note : La petite taille de certains des éléments microscopiques et la présence d'un épithécium ont rendu les mesures et les observations difficiles à préciser.

Stip Helleman, 11-04-2013 08:13

Re : Orbillia sp. - 50466

Hi Roland,
this collection strongly resembles Hyalorbilia inflatula, the asci look like arising from croziers (Hyalorbilia) on Jacqeline's photostream. But perhaps Zotto has a different idea

best wishes,
Stip

Hans-Otto Baral, 11-04-2013 11:03

Re : Orbillia sp. - 50466

Hi Roland & Stip

Stip is right with Hyalorbilia, the asci have a rather wide, unstalked base  and certainly arise from croziers, unlike Orbilia. Also the excipulum is of a horizontal prismatica.

But H. inflatula has straight spores (or almost so). We have H. tortuosa and  H. fagi as the two species in question, both undescribed, and at least the latter quite frequent. But the two can not easily be separated. The best is when you find the conidia on the substrate (or in culture), they are quite different. From your narrow spore width H. tortuosa would better fit. Could you please send me spore photos with an included scale?

I attach a part of my key, so you can try whether you arrive at tortuosa.

The margin of your schematic section is not well figured, it needs to look round and a bit inrolled rather than cut. And the photo on the miidle left is misleading, it does not show a section but a squash mount. It pretends that the cells are oriented perpendicular to the surface while they run in fact parallel to the surface.
 
Zotto

• Key-to-species-of-Hyalorbilia-0001.doc

Hans-Otto Baral, 11-04-2013 18:09

Re : Orbillia sp. - 50466

The key should include images, I know.

Here a part of the H. inflatula plate where you see that the spores are generally straight or almost so.  

Also plates of fagi and tortuosa in the documents below

Zotto

• 

• H.-fagi-150-0001.jpg
• H.-fagi-300-0001.jpg
• H.-texensis-300-0001.jpg
• H.-tortuosa-150-0001.jpg
• H.-inflatula1-300-0001.jpg

Hans-Otto Baral, 11-04-2013 18:14

Re : Orbillia sp. - 50466

One plate was wrong, here the right one

• H.-tortuosa-300-0001.jpg

Hans-Otto Baral, 12-04-2013 07:29

Re : Orbilia sp. - 50466

Hi Roland

your spores partly look like inflatula but others are too much curved. I never saw inflatula like that. I know it is a very difficult group, and perhaps intermediate cases exist. We have a few records we cannot safely place. Regrettably we have much too few sequences to get a better idea.

If you saw any conidia (~30-40 x 4-5 µm, multiseptate) in your collection this would be very helpful.

Could you please tell me  the locality where it comes from?

Thanks
Zotto

Hans-Otto Baral, 14-04-2013 22:22

Re : Orbilia sp. - 50466

o.k., this is a rather rough hint, it is only the county.  In any case, for a citation the finder and herbarium would also be necessary.

Zotto

Hans-Otto Baral, 15-04-2013 08:08

Re : Orbilia sp. - 50466

Thanks, Roland! 

Lac Beauport lies at 46° 56' N 71° 58' W at ca. 250 m alt.  Now, Jules Simon found it close to his home? And the number is his personal herbarium I think.

Zotto