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04-11-2025 12:43

Edvin Johannesen Edvin Johannesen

Hi! One more found on old Populus tremula log in O

04-11-2025 09:07

Josep Torres Josep Torres

Hello.A suspected Hymenoscyphus sprouting on a thi

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Josep Torres Josep Torres

Hello.Very small, globose, mucronate perithecia, b

03-11-2025 21:34

Edvin Johannesen Edvin Johannesen

These tiny (0.4-0.5 mm diam.), whitish, short-stip

03-11-2025 19:41

David Chapados David Chapados

Hi,Does anyone knows which genus could this be? G

28-10-2025 15:37

Carl Farmer

I'd be grateful for any suggestions for this strik

03-11-2025 16:30

Hans-Otto Baral Hans-Otto Baral

Hello I want to ask you if you have found this ye

01-11-2025 09:14

Francis Maggi

Bonjour,Trouvé sur Xanthoria parietina à Valdebl

28-10-2025 19:33

Nicolas Suberbielle Nicolas Suberbielle

Bonjour à tous,Je voudrais votre avis sur cette r

31-10-2025 09:19

Lothar Krieglsteiner Lothar Krieglsteiner

Can somebody provide me with a file of:Rogerson CT

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Paraphyses IKI reaction in Scutellinia
Perz Piotr, 03-02-2007 23:10
Hi,

I have question about paraphyses IKI reaction in genus Scutellinia. Sometimes paraphyses stain in IKI green - it means they have carotenoids. Sometimes there is no IKI reaction - also paraphyses does not contains carotenoids.

How is the name for violet-lila reaction? Here paraphyses of Scutellinia pseudotrechispora (Schröt.) Le Gal in IKI

  • message #1406
NC NC, 04-02-2007 10:10
Re:Paraphyses IKI reaction in Scutellinia
Bonjour,
J'ai déjà observé cette réaction violette avec le Lugol par exemple avec Scutellinia crucipila. Je pense que la réaction (carotene à l'extrémité des paraphyses)en vert foncé (vert-bouteille) s'arrete par manque de concentration en iode (??) et le mélange orange + vert foncé donne cette couleur lilas. Dans ton cas une préparation sans eau, seulement avec IKI donne t-elle toujours du violet?
Amicalement
Robert
Hans-Otto Baral, 04-02-2007 11:54
Hans-Otto Baral
Re:Paraphyses IKI reaction in Scutellinia
I have actually observed the same in "Scutellinia sp." (see my paper on Lugol versus Melzer, mycotaxon 29: 428, 1987): when adding IKI (Lugol) to a water mount, the colour reaction of the paraphyses changes from greyish-violet to grey.olive or grey-brown. The violet reaction is seen at an iodine concentration of 0.05-0.1% while the olive appears at 0.1-0.2 % and higher. So the colour is concentration-dependent similar as in the hemiamyloid apical rings. But contrary to hemiamyloidity, the violet and olive are also seen when KOH-pretreated, and I believe also in Melzer.

Zotto
Perz Piotr, 11-02-2007 09:00
Re:Paraphyses IKI reaction in Scutellinia
Thanks a lot, Robert & Zotto!