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07-05-2025 18:29

Francois Guay Francois Guay

I found this small hyaline ascomycete on moist, de

05-05-2025 09:35

Nicolas Suberbielle Nicolas Suberbielle

Bonjour à tous,Marie-Rose d'Angelo de la SociétÃ

07-05-2025 10:18

Thomas Flammer

I have found on Genistae sth. that looks like puli

06-05-2025 13:37

Thomas Flammer

Munk, Anders (1953). The System of the Pyrenomycet

06-05-2025 12:52

Castillo Joseba Castillo Joseba

Me mandan el material de Galicia (España),  reco

06-05-2025 13:22

Castillo Joseba Castillo Joseba

Me mandan el material de Galicia (España),  reco

05-05-2025 22:06

Patrice TANCHAUD

Bonsoir, il y a quelques temps, j'avais évoqué

01-05-2025 20:11

Zuzana Sochorová (Egertová) Zuzana Sochorová (Egertová)

Hello, today I have collected a tiny ascomycete g

27-04-2025 15:54

Martin Bemmann Martin Bemmann

Can somebody provide this article from a Leningrad

03-05-2025 18:35

Edmond POINTE Edmond POINTE

J'ai  connu Michel DELPONT au début de ma passio

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Paraphyses IKI reaction in Scutellinia
Perz Piotr, 03-02-2007 23:10
Hi,

I have question about paraphyses IKI reaction in genus Scutellinia. Sometimes paraphyses stain in IKI green - it means they have carotenoids. Sometimes there is no IKI reaction - also paraphyses does not contains carotenoids.

How is the name for violet-lila reaction? Here paraphyses of Scutellinia pseudotrechispora (Schröt.) Le Gal in IKI

  • message #1406
NC NC, 04-02-2007 10:10
Re:Paraphyses IKI reaction in Scutellinia
Bonjour,
J'ai déjà observé cette réaction violette avec le Lugol par exemple avec Scutellinia crucipila. Je pense que la réaction (carotene à l'extrémité des paraphyses)en vert foncé (vert-bouteille) s'arrete par manque de concentration en iode (??) et le mélange orange + vert foncé donne cette couleur lilas. Dans ton cas une préparation sans eau, seulement avec IKI donne t-elle toujours du violet?
Amicalement
Robert
Hans-Otto Baral, 04-02-2007 11:54
Hans-Otto Baral
Re:Paraphyses IKI reaction in Scutellinia
I have actually observed the same in "Scutellinia sp." (see my paper on Lugol versus Melzer, mycotaxon 29: 428, 1987): when adding IKI (Lugol) to a water mount, the colour reaction of the paraphyses changes from greyish-violet to grey.olive or grey-brown. The violet reaction is seen at an iodine concentration of 0.05-0.1% while the olive appears at 0.1-0.2 % and higher. So the colour is concentration-dependent similar as in the hemiamyloid apical rings. But contrary to hemiamyloidity, the violet and olive are also seen when KOH-pretreated, and I believe also in Melzer.

Zotto
Perz Piotr, 11-02-2007 09:00
Re:Paraphyses IKI reaction in Scutellinia
Thanks a lot, Robert & Zotto!