Accès membres

Mot de passe perdu? S'inscrire

16-05-2025 20:59

Jean-Paul Priou Jean-Paul Priou

Bonsoir à tous, un ami normand vient de me faire

16-05-2025 05:47

Francois Guay Francois Guay

I found this super tiny hyaline asco on fir needle

11-05-2025 10:35

ruiz Jose

Hola, en excremento de jabali, tamaño de unos 2 m

16-05-2025 13:12

Castillo Joseba Castillo Joseba

Me mandan el material de Galicia (España),  reco

14-05-2025 15:08

Gernot Friebes

Hi,I'd appreciate your help with this Lachnum coll

16-05-2025 07:36

Zhuo Lan

I am a Ph.D. candidate in fungal taxonomy at Capit

14-05-2025 10:57

Nicolas Suberbielle Nicolas Suberbielle

Bonjour,Encore une trouvaille de Marie-Rose D'Ange

13-05-2025 12:32

Gernot Friebes

Hi,I found this one on a Picea abies branch. It lo

08-07-2023 19:24

Juuso Äikäs

These Mollisia fruitbodies were growing on a twig

12-05-2025 18:25

Thomas Flammer

Substrate rabbitSpores: 12-13 µm x 6-7 µmParaph

« < 1 2 3 4 5 > »
Eutypa lejoplaca?
Garcia Susana, 23-01-2014 14:32
Hi all

I found this Pyrenomycete, growing on wood of Acer campestre.
Perithecia size is 200-260um in diameter. Ostioles are 115-140um in diameter and have a cruciform shape.
Asci measured 55-68 × 5-7um, area spores 15-23 × 5-6.5um. With IKI, the apical apparatus becomes more apparent, but I'm not able to say if it is IKI +. in any case would be weakly positive.
Persistent paraphyses.

Following keys Rappaz, came to Eutypa Lejoplaca but I doubt if it could be another species, such as E. maura, or leptoplaca (if I consider the reaction to IKI is +).


Can anyone identify the species with the data provided?


Thank you,


Susana (Spain)

  • message #27030
  • message #27030
  • message #27030
  • message #27030
Jacques Fournier, 23-01-2014 17:49
Jacques Fournier
Re : Eutypa lejoplaca?
Hola Susana,
you are right, your fungus is likely around E. leptoplaca and E. lejoplaca. Eutypa maura can be ruled out because it is distinctive in having more immersed perithecia and the wood markedly blackened beneath the surface between the necks.
The most reliable difference between the two species is the reaction of the apical apparatus to iodine.
I suggest you follow Rappaz's procedure with a short pretreatment of perithecial content in 3% KOH and mounting in Melzer's reagent. This leads to a more drastic retraction of the ascal content than IKI and makes the apical apparatus easier to make out. Remember it is in fact subapical, at the bottom of a shallow tubular structure. The thing is to work on a very small amount of material in order to make a very thin slide, otherwise interpreting the iodine reaction is most often tricky, especially in species where the apical apparatus is very small. This applies to all species of Diatrypaceae since the character of reaction to iodine is crucial.
Come back with a clear result, whatever how long it takes!
Cheers,
Jacques
Garcia Susana, 23-01-2014 21:50
Re : Eutypa lejoplaca?
Salut Jacques

¿C'est la réaction tout aussi intense avec KOH + Melzer, qui KOH + IKI?


J'ai fait quelques préparations KOH + IKI (je n'ai pas de Melzer) et pense que c'est positif, j'observe dans les asques un couple de très petits points que je dirais sont bleus.


Que pensez-vous?


Saludos


Susana

  • message #27050
Jacques Fournier, 24-01-2014 08:56
Jacques Fournier
Re : Eutypa lejoplaca?
Hola Susana,
ton excellente photo montre bien une réaction positive, amyloide. Tu peux donc écarter lejoplaca.
E. leptoplaca est beaucoup plus fréquente sur Fraxinus, tu devrais la trouver facilement sur des petites branches tombées récemment avec le vent et tu pourras ainsi lui comparer ta récolte.
Amitiés,
Jacques
Garcia Susana, 24-01-2014 11:04
Re : Eutypa lejoplaca?
Muchas gracias Jacques

Saludos.
Susana