20-05-2014 17:57
Nicolas VAN VOOREN
... d'une plante herbacée (peut-être une Poaceae
20-05-2014 18:32
Alain BRISSARDBonsoir à tousDécidèment je n'arrive pas à êt
21-05-2014 00:27
Malcolm Greaves
This was found on very wet rotted wood.The spores
20-05-2014 13:23
Lepista ZacariasDear all,I need your help to try to identify what
19-05-2014 09:01
Dragiša SavicHi everyone. This Pleospora species I found on the
18-05-2014 22:25
Lepista ZacariasJe serai très reconnaissant si quelqu'un d'entre
07-05-2014 22:00
Bernard Declercq
Good evening, Collected on bark of Salix cinerea:
... d'une plante herbacée (peut-être une Poaceae).Apothécies 0.4-1 mm diam. Marge nettement villeuse.
Poils marginaux clavés dans l'article sommital, distinctement incrustés, 28-46 × 7-10 µm (partie la plus large). Asques sans crochet, 62-70 × 6-7 µm, pars spor. 29-33 µm, anneau apical IKI+ (bb). Paraphyses × 3–4.5 µm. Ascospores 11.5-16 (17) × 1.8-2.5 µm, fusiformes étroites, souvent courbées, contenant plusieurs guttules.
Je n'arrive à rien de concluant en utilisant les clés de A. Gminder ou de Zotto.
Une idée ?
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Apothecia 0.4-1 mm diam. Margin distinctly villose.
Marginal hairs clavate in the top article, distinctly incrusted, 28-46 × 7-10 µm (wider part). Asci without crozier, 62-70 × 6-7 µm, pars spor. 29-33 µm, apical ring IKI+ (bb). Paraphyses × 3–4.5 µm. Ascospores 11.5-16 (17) × 1.8-2.5 µm, narrow fusoid, often curved, containing several oil-drops.
Did you test KOH?
Zotto
I've collected a similar-looking species from Aberystwyth, Wales, UK from Cortaderia (pampas grass), as part of my PhD thesis.
Based on the ITS DNA sequence my collection seems to be a close relative of what I consider to be Mollisia cf. poaeoides, which colonises turf-grass culms around Aberystwyth and possesses shorter ascospores with similar oil droplets. I suspect the ascospore size difference in the larger-spored species is associated with a host-jump to another larger grass species.
At the moment, it looks like there are many closely related species in this group that are endophytes of grass, orchid and other hosts (based on isolates and environmental sequences from plants). None of these have been collected and sequenced yet, so it is possible that there are other species on similar hosts with similar morphology.
Checking through my images I also can't see any distinct croziers, but I wasn't specifically looking for them when taking the images, and also wouldn't consider this a diagnostic feature at the moment. I didn't observe any KOH reaction.
I've attached a slide of some of my images so you can compare with your collection.
Cheers,
Brian
Mollisia AB1
Be sure that the absence of croziers is an extremely important character, certainly constant within a species of Mollisia or Pyrenopeziza.
In Mollisia I recall M. rosae and M. asteroma as having always simple septate asci. M. prunicola and M. excelsior might be confused with their respective counterparts, but alsways have croziers.
On your photo I cannot see well an ascus base.
Nicolas, did you look for crystals? I have the suspicion that you fungus could be M. retincola, though I have noted a larger spore size in my files.
Zotto
I made a new mount to check some points:
1) KOH-reaction: faintly yellow
2) No crystal seen
3) Crozier present. I didn't see them first time because they seem to be placed very low in the ascogenous hyphae.
I now compare yours with M. luctuosa = humidicola. That species has 1-septate spores insider the living asci, however, though I am not sure about this in every of the specimens I have in my folder. The macroscopy is very similar at least.
Zotto
I suggest alternatively to make a Melzer mount in which septa are often rather striking. If you find enough asci with septate spores inside this would be a further confirmation for luctuosa.
Good tip :-)
Zotto - I've emailed you regarding my issues with the taxonomic value of croziers (at least in Mollisia and Pyrenopeziza). Hopefully I'm just being stupid and you can correct me!
Cheers,
Brian