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S. capillipes
Hans-Otto Baral,
06-06-2025 12:12
Dear all
I want to ask you if you have any recent, well-preserved collection of "Sclerotinia" capillipes. My paper on this species is almost finished, but its molecular part is based on only one ITS sequence of a 4-spored collection. Either a second sequence from a 4-spored find would be welcome, or better a sequence from an 8-spored specimen. May-June is the best season for it, so whe you are in an alder swamp please have a look on rotten Alnus leaves. Although 4- and 8-spored apothecia may occur simultaneously at the same place, there is still unclear if they are the same species.
In case of success, the apothecia must be checked for their spore number before they can be sequenced.
Thanks
Zotto
David Malloch,
06-06-2025 14:22
Re : S. capillipes
Hi Zotto,
Could you post a picture of this species? We have lots of alder wetlands where I live and I could see if it's here.
Dave
Hans-Otto Baral,
06-06-2025 17:24
Re : S. capillipes
Good question. You can loook here:
and under this address is a link to a paper by Uwe Lindemann:
David Malloch,
06-06-2025 17:32
Re : S. capillipes
Thanks Zotto. Those are excellent photos. If I find it here I'll let you know.
Hans-Otto Baral,
06-06-2025 17:38
Re : S. capillipes
Quite characteristic is the somewhat unshaped, angular or banana-like sclerotium. Other species on alder leaves have only a stroma but no sclerotium. The sclerotium is still attached to the rotten leaf, but the leaf may be so fragmentated that the host is +/- unrecognizable.
Louis DENY,
15-06-2025 17:27
Re : S. capillipes
Zotto,
I found this species which is maybe what you're looking for.
Close to Belfort, east of France, altitude 400m, in a very wet place, under Alnus glutinosa, Apothecie diameter 0,5 - 1,5 mm, very long stipe, up to more than 2-3 cm, From a clerote,
Ascus 8 spores, 80 - 90 X 6-7, lugol weak,
Spores 2,8-3,5 X 8-9, OCI = 0, I think unicellular,
Texture globulosa,
What perturbates me is that 2 weeks ago, at the very same place I found exemplar with ascus with 4 spores. So i'm not 100% sure.
My micro photo are note very good, but I wanted not to use all the specimen
I found this species which is maybe what you're looking for.
Close to Belfort, east of France, altitude 400m, in a very wet place, under Alnus glutinosa, Apothecie diameter 0,5 - 1,5 mm, very long stipe, up to more than 2-3 cm, From a clerote,
Ascus 8 spores, 80 - 90 X 6-7, lugol weak,
Spores 2,8-3,5 X 8-9, OCI = 0, I think unicellular,
Texture globulosa,
What perturbates me is that 2 weeks ago, at the very same place I found exemplar with ascus with 4 spores. So i'm not 100% sure.
My micro photo are note very good, but I wanted not to use all the specimen
Hans-Otto Baral,
15-06-2025 17:47
Re : S. capillipes
Dear Louis!
This is exactly what I was asking for. And great that you found the 4-spored form as well.
Please air-dry both.
Now the problem is that we must be fully sure if the new sample is always 8-spored. Is anything left of the apo that you examined?
Now I must clarify who will be going to take a sequence.
Could you please tell me the date and perhaps coordinates?
Thanks so much!
Zotto
Louis DENY,
15-06-2025 18:27
Re : S. capillipes
Dear Zotto,
They were found today, GPS: 47.6815 __6.86697
Nothing left from the apo i examined. I have 3 or 4 apos in the fridge and I left a couple additional where I found them. (no problem to go back there, if needed)
I found the 4 spores exemplary 2 weeks ago and I do not keep exicata.
I have no idea if the exemplaries found today are all 8 spores Do you want me to check that?
Hans-Otto Baral,
15-06-2025 20:48
Re : S. capillipes
Perhaps you can take out a very small piece of hymenium with a sharp needle. After microscopic check put the apo in a paper bag for drying. You can repeat this with the another apo.
Principally one apo is enough for a sequence, tow might be better, but both must be checked.
Did you photograph anything of the 4-spored one? Which date was that?If you know a lab in France, this would be the easiest. I guess that the French mycological society is interested on such rare finds. I was once in contact with Jean-Michel Bellanger (Mycoseq) regarding Luteodiscus.
Louis DENY,
15-06-2025 21:41
Re : S. capillipes
No photo, but I already posted a request on this forum some years ago for a 4 spores version: http://ascofrance.fr/search_forum/63362
I'll do the check for other apos tommorow and keep you informed.
I don't have any contact with a lab. I'll try to find one
Hans-Otto Baral,
15-06-2025 21:49
Re : S. capillipes
Yes, I knew about this, 7.5 km NW of Belfort, Etang de Chenebier. Now 4.7 km N of Belfort.
So I assume you found the 4-spored one on 1. June 2025.
Louis DENY,
15-06-2025 21:58
Re : S. capillipes
Correct: 01/06/2025, same place as today
Louis DENY,
16-06-2025 10:30
Re : S. capillipes
I have 4 apos.
I tried to determine the number of spores per ascus. Quite difficult exercise because they are in a bad shape and it's almost impossible to find ascus with their spores.
3 are obviously 8 spore version.
For the last one I finally found 1 ascus, which has 4 spores only, but being alone, I'm not sure that the result is significant
I tried to determine the number of spores per ascus. Quite difficult exercise because they are in a bad shape and it's almost impossible to find ascus with their spores.
3 are obviously 8 spore version.
For the last one I finally found 1 ascus, which has 4 spores only, but being alone, I'm not sure that the result is significant
Hans-Otto Baral,
16-06-2025 10:36
Re : S. capillipes
o.k., they seem to have shot most of their spores. Then I would only take those for DNA where you saw 8 spores. Please let them dry out immediately to avoid contamination.