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13-12-2025 11:58

Mirek Gryc

HiSupposedly this is a species that occurs quite o

12-12-2025 18:39

Mirek Gryc

Hello everyone.Macrofeatures similar to Mollisia b

09-12-2025 12:06

Andgelo Mombert Andgelo Mombert

Bonjour,Je recherche l'article concernant Hypobryo

07-12-2025 16:07

Arnold Büschlen

Hallo, ich habe in einer Moos-Aufsammlung (epiphy

08-12-2025 21:04

Mark Stevens

"Hello everyone,I'm relatively new to microscopy (

08-12-2025 18:59

Lothar Krieglsteiner Lothar Krieglsteiner

.. found by a seminar-participant, I do not know t

08-12-2025 21:18

Buckwheat Pete

Hello everyone, Is it possible to at least approx

07-12-2025 17:43

Malcolm  Greaves Malcolm Greaves

This Helvella was in mixed woodland. Uniform cupul

08-12-2025 17:37

Lothar Krieglsteiner Lothar Krieglsteiner

20.6.25, on branch of Abies infected and thickened

16-03-2014 22:00

Ralph Vandiest Ralph Vandiest

Hello,I found this species a few months ago but ha

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Orange disks
Leif Goodwin, 14-10-2023 11:29
This summer I found some cup fungus on soil with acer and black poplar. I struggled to get a spore print so only got a few spores. The asci and paraphyses are shown in Melzers, the spores in Lacto Cotton Blue. The disks are up to 2 cm wide. 

Someone suggested a Purpureodiscus species e.g. P. isabellinus. 

Help appreciated.
  • message #77143
  • message #77143
  • message #77143
Nicolas VAN VOOREN, 22-10-2023 11:19
Nicolas VAN VOOREN
Re : Orange disks
Macroscopically it looks like Purpureodiscus subisabellinus, but you should show some mature ascospores (in water). They measure 18.5-23.5 × 11-13 µm and show bipolar spore granules. The amyloid reaction of asci is only visible on the wall (W type), sometimes difficult to see.
Leif Goodwin, 13-11-2023 18:43
Re : Orange disks
Thank you for taking the time to reply, and apologies for my late response. I have given the specimen to someone who will amplify the ITS region of the DNA and then have it sequenced. When he returns it, I will try and find some mature spores. Hopefully the DNA sequencing will give us an answer, but sadly DNA does not always amplify, possibly because the primers are not ideal for ascos.