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17-09-2025 19:43

Philippe PELLICIER

Sur branche morte de Mélèze. Les ascospores sphÃ

18-09-2025 08:35

Edmond POINTE Edmond POINTE

Bonjour amis mycologues,Trouvé sur moquette de ch

17-09-2025 16:14

Philippe PELLICIER

Apothécies enterrées, fermées au début puis s'

17-09-2025 10:50

Heather Merrylees

Hi there!I am hoping for any advice on the identif

11-09-2025 16:57

Jason Karakehian Jason Karakehian

Our revision of Marthamycetales (Leotiomycetes) is

16-09-2025 12:53

Philippe PELLICIER

Pézizes de 1-4 mm, brun grisâtres, sur les capsu

03-09-2025 12:44

Enrique Rubio Enrique Rubio

Hi to somebody.I would like to know your opinion o

15-09-2025 14:40

Nicolas VAN VOOREN Nicolas VAN VOOREN

Hello.I'm searching for a digital copy of the seco

14-09-2025 22:16

Philippe PELLICIER

Apothécies petites jusquà 3 mm, oranges, avec de

13-09-2025 14:01

Thomas Flammer

dark brown apothecia, splitIKI-Spores biguttulate

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Orange disks
Leif Goodwin, 14-10-2023 11:29
This summer I found some cup fungus on soil with acer and black poplar. I struggled to get a spore print so only got a few spores. The asci and paraphyses are shown in Melzers, the spores in Lacto Cotton Blue. The disks are up to 2 cm wide. 

Someone suggested a Purpureodiscus species e.g. P. isabellinus. 

Help appreciated.
  • message #77143
  • message #77143
  • message #77143
Nicolas VAN VOOREN, 22-10-2023 11:19
Nicolas VAN VOOREN
Re : Orange disks
Macroscopically it looks like Purpureodiscus subisabellinus, but you should show some mature ascospores (in water). They measure 18.5-23.5 × 11-13 µm and show bipolar spore granules. The amyloid reaction of asci is only visible on the wall (W type), sometimes difficult to see.
Leif Goodwin, 13-11-2023 18:43
Re : Orange disks
Thank you for taking the time to reply, and apologies for my late response. I have given the specimen to someone who will amplify the ITS region of the DNA and then have it sequenced. When he returns it, I will try and find some mature spores. Hopefully the DNA sequencing will give us an answer, but sadly DNA does not always amplify, possibly because the primers are not ideal for ascos.