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31-12-2025 19:27

Spooren Marco Spooren Marco

Collected from loamy soil, at waterside (completel

29-12-2025 17:51

Blasco Rafael Blasco Rafael

Hola, me pueden ayudar con esta muestra.Recogida s

30-12-2025 16:44

Pascal Ducos

Bonjour,Une anamorphe rose stipitée, très nombre

29-12-2025 23:20

éric ROMERO éric ROMERO

Bonjour, Une récolte du mois d'août 2025 en tou

30-12-2025 17:14

Bernard CLESSE Bernard CLESSE

Bonjour à toutes et tous,Pourriez-vous aider Albe

30-12-2025 15:31

Johan Boonefaes Johan Boonefaes

I found this unknown star form by the microscopy o

29-12-2025 10:15

Hulda Caroline Holte

Hello, I found and collected this propoloid ascom

30-12-2025 09:04

Josep Torres Josep Torres

Hello.A Pyrenomycete sprouting sparsely but very d

29-12-2025 17:44

Isabelle Charissou

Bonjour,J'aimerais savoir si d'autres personnes au

12-11-2021 00:03

Lepista Zacarias

Hi everybody,A week ago in my fiels trip I noticed

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Whitish inoperculate on the liverwort Aneura pinguis
Edvin Johannesen, 25-03-2023 14:42
Edvin JohannesenI have received this dried specimen for microscopy. In the fresh state the apothecia are (purplish-greyish) white, but they become black in the dry state (see photos of both states). On rehydration they appear somewhat gelatinous, dark purplish brown.
Hymenium is a brown, dense palisade. Excipulum is paler brown, I would say textura epidermoidea (?). Asci and spores appear dead. Mature spores are brown, 20 x 6 micr., ellipsoid/allantoid, aseptate or I suspect becoming 1-septate (hard to see).  Asci IKI negative. 
All micro images in water.
I have not been able to find any bryophilous inoperculates with such brown spores.

Any suggestions?
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Georges Greiff, 29-03-2023 13:03
Re : Whitish inoperculate on the liverwort Aneura pinguis
Very interesting collection. I have not seen any inoperculates on liverworts with brown spores. It may well be undescribed. Bryoscyphus is superficially similar and seeing living paraphyses would have helped. Bryoscyphus conocephali agg. is on liverworts in similar habitats. It is not that but it might be related.

George
Edvin Johannesen, 29-03-2023 14:55
Edvin Johannesen
Re : Whitish inoperculate on the liverwort Aneura pinguis
Thanks for your opinion, Georges.  I will have another attempt at looking for paraphyses (I doubt they will be alive). I assume that the spore color is not a result of necrosis or some other process?
Georges Greiff, 29-03-2023 15:19
Re : Whitish inoperculate on the liverwort Aneura pinguis
Dear Edvin,

I have not noted brownish spores in any of the herbarium material of Bryoscyphus that I have examined, but that doesn't mean it won't happen. The darkening of the dry, old apothecia is a common occurrence Bryoscyphus, including in B. conocephali I collected recently. Perhaps this pigment has gone into the spores. I can check if this darkening happens in spores of my B. conocephali.

Unfortunately the paraphyses will be dead so you won't see the VBs. Perhaps now only sequencing can place this collection with more certainty within one of the bryophilous Leotiomycete groups. They are tricky even when fresh.

All the best,
George
Edvin Johannesen, 29-03-2023 15:40
Edvin Johannesen
Re : Whitish inoperculate on the liverwort Aneura pinguis
It would be great if you could check this - thanks!