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Bonjour à toutes et tous,Pourriez-vous m'aider à

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ruiz JoseHola, en excremento de jabali, tamaño de unos 2 m
Black apothecia with greenish septate spores on Picea abies twig
Edvin Johannesen,
23-03-2023 12:36

Asci are 4-spored, clavate with a very long, alomost hypha-like base, ca. 20 micr. across at their broadest. Paraphyses filiform, ca. 1 micr. thick.
Spores 3-septate, greenish at maturity, 25-30 x 9-10 micr.
Asci stain blue in Lugol, somewhat stronger towards the apex. Asci also weakly blue in Lugol after pre-treatment with KOH and water rinsing. Spores possibly also somewhat amyloid, but masked by the initial green color of spore wall.
Are we in Ostropales? In the "vicinity" of Tryblidiopsis? Opinions appreciated. Thanks!
Edvin Johannesen,
23-03-2023 12:37
Edvin Johannesen,
23-03-2023 12:38
Edvin Johannesen,
23-03-2023 12:39
Edvin Johannesen,
23-03-2023 12:40
Hans-Otto Baral,
23-03-2023 16:53

Re : Black apothecia with greenish septate spores on Picea abies twig
This reminds me of Lahmia kunzei = Parkerella populi, which is known from POpulus bark with much smaller apos (0.2-0.3 mm), 8-spored inamyloid asci, and apparently thin-walled, allantoid, 3-septate spores of 18-37 x 4-5. Only 1 species is known on the genus.
I have it in Tympanidaceae, but this is tentative and no sequence is known to me.
Edvin Johannesen,
23-03-2023 17:04

Re : Black apothecia with greenish septate spores on Picea abies twig
Thanks! So I would say, despite similarities, quite different from Lahmia kuntzei. Jason K. has offered to sequence it and perhaps also try to cultivate it. Do the spores look dead, by the way?
Hans-Otto Baral,
23-03-2023 17:15

Re : Black apothecia with greenish septate spores on Picea abies twig
The multiguttulate ones are alive.
Jason Karakehian,
23-03-2023 19:15
Re : Black apothecia with greenish septate spores on Picea abies twig
We should tell Luis Quijada about this if you think Tympanidaceae. The caespitose apothecia seem to suggest that, but I have no experience with the group.
Edvin Johannesen,
23-03-2023 21:51

Re : Black apothecia with greenish septate spores on Picea abies twig
OK, thanks again. I have been in touch with Luis a couple of times before, so I will bring this to his attention.