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13-05-2025 12:32

Gernot Friebes

Hi,I found this one on a Picea abies branch. It lo

08-07-2023 19:24

Juuso Äikäs

These Mollisia fruitbodies were growing on a twig

12-05-2025 18:25

Thomas Flammer

Substrate rabbitSpores: 12-13 µm x 6-7 µmParaph

02-05-2025 10:16

Bernard CLESSE Bernard CLESSE

Bonjour à toutes et tous,Pourriez-vous m'aider à

12-05-2025 18:52

Gonzalez Garcia Marta

Buenas tardes, estoy estudiando un hongo peritecio

10-05-2025 18:47

David Chapados David Chapados

Hi, Does anyone have an idea of what this could b

11-05-2025 17:57

Louis DENY

Hello forumTrouvé dans les environs de Belfort,al

08-05-2025 20:50

Andreas Jacob

Good evening, due to contstant drought I started

11-05-2025 10:22

Karl Soler Kinnerbäck

Found on moist Betula wood dipped in freshwater st

11-05-2025 10:35

ruiz Jose

Hola, en excremento de jabali, tamaño de unos 2 m

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Black apothecia with greenish septate spores on Picea abies twig
Edvin Johannesen, 23-03-2023 12:36
Edvin JohannesenThese black, erumpent, initially closed apothecia were found in small groups on a thin twig of an old spruce tree in February, in a mountainous area in central-southern Norway. They eventually open and disclose a yellowish white hymenium. The spores are greenish, which can be seen even in reflected light. The largest, open apothecium measure 2 mm.
Asci are 4-spored, clavate with a very long, alomost hypha-like base, ca. 20 micr. across at their broadest. Paraphyses filiform, ca. 1 micr. thick.
Spores 3-septate, greenish at maturity, 25-30 x 9-10 micr. 
Asci stain blue in Lugol, somewhat stronger towards the apex. Asci also weakly blue in Lugol after pre-treatment with KOH and water rinsing. Spores possibly also somewhat amyloid, but masked by the initial green color of spore wall.
Are we in Ostropales? In the "vicinity" of Tryblidiopsis?  Opinions appreciated.  Thanks!
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Edvin Johannesen, 23-03-2023 12:37
Edvin Johannesen
Re : Black apothecia with greenish septate spores on Picea abies twig
Asci and spores in water.
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Edvin Johannesen, 23-03-2023 12:38
Edvin Johannesen
Re : Black apothecia with greenish septate spores on Picea abies twig
Asci and spores in Lugol (no pre-treatment).
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Edvin Johannesen, 23-03-2023 12:39
Edvin Johannesen
Re : Black apothecia with greenish septate spores on Picea abies twig
Ascus in Lugol after KOH pre-treatment/rinsing.
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Edvin Johannesen, 23-03-2023 12:40
Edvin Johannesen
Re : Black apothecia with greenish septate spores on Picea abies twig
Ascus and spores in water.  Phase contrast to show septation.
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Hans-Otto Baral, 23-03-2023 16:53
Hans-Otto Baral
Re : Black apothecia with greenish septate spores on Picea abies twig
This reminds me of Lahmia kunzei = Parkerella populi, which is known from POpulus bark with much smaller apos (0.2-0.3 mm), 8-spored inamyloid asci, and apparently thin-walled, allantoid, 3-septate spores of 18-37 x 4-5. Only 1 species is known on the genus.

I have it in Tympanidaceae, but this is tentative and no sequence is known to me.
Edvin Johannesen, 23-03-2023 17:04
Edvin Johannesen
Re : Black apothecia with greenish septate spores on Picea abies twig
Thanks! So I would say, despite similarities, quite different from Lahmia kuntzei. Jason K. has offered to sequence it and perhaps also try to cultivate it. Do the spores look dead, by the way?
Hans-Otto Baral, 23-03-2023 17:15
Hans-Otto Baral
Re : Black apothecia with greenish septate spores on Picea abies twig
The multiguttulate ones are alive.
Jason Karakehian, 23-03-2023 19:15
Jason Karakehian
Re : Black apothecia with greenish septate spores on Picea abies twig
We should tell Luis Quijada about this if you think Tympanidaceae. The caespitose apothecia seem to suggest that, but I have no experience with the group.
Edvin Johannesen, 23-03-2023 21:51
Edvin Johannesen
Re : Black apothecia with greenish septate spores on Picea abies twig
OK, thanks again. I have been in touch with Luis a couple of times before, so I will bring this to his attention.