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Unguiculella eurotioides?
Riet van Oosten,
05-03-2023 19:54
Hello,
Found by Laurens van der Linde, March 2023 on Rubus.
Could this be Unguiculella eurotioides?
With croziers?
MLZ-
Greetings, Riet
Stip Helleman,
05-03-2023 20:10
Re : Unguiculella eurotioides?
Hi Riet,
indeed with croziers, the ascus dimensions fit to hamulata but the spores with the two guttules fits to no so far described species. It fits to the undiscribed Spanish collection in Zotto's sambucus folder
cheers,
Stip
Riet van Oosten,
05-03-2023 20:52
Re : Unguiculella eurotioides?
Thank you very much for your help and the information Stip!
Hans-Otto Baral,
06-03-2023 09:33
Re : Unguiculella eurotioides?
Are these glassy hairs 5% KOH-resistent? Did you test IKI as well?
Riet van Oosten,
06-03-2023 14:34
Re : Unguiculella eurotioides?
I also tested IKI but it was difficult to see.
Today another try (IKI-) and also the KOH test, but I only have KOH 3 % :-(
Hans-Otto Baral,
06-03-2023 15:54
Re : Unguiculella eurotioides?
Clearly inamyloid.
With 3% it is not a problem, but: you must hold the preparation over a flame until it shortly starts boiling. Then you have the same effect or even better than with 5% KOH.
The solid glassy hairs exclude an Unguiculella as I know the genus. I did not compare so far where it could belong.
I also wonder if you measured the spores. Stip is right in comparing the find on Sambucus.
Riet van Oosten,
06-03-2023 20:40
Re : Unguiculella eurotioides?
I hope I did it right ... I heated the preparation with the coverglass upon it.
Spore sizes .... difficult to measure
Hans-Otto Baral,
06-03-2023 20:59
Re : Unguiculella eurotioides?
Good, but you need to take a detail of your KOH photos at larger scale to see if the glassy substance resisted. Thanks for the spores. If your photos are so small as we see them, measuring is difficult indeed.
Riet van Oosten,
06-03-2023 21:18
Re : Unguiculella eurotioides?
Another try :-)
Stip Helleman,
06-03-2023 23:24
Re : Unguiculella eurotioides?
Spores are measured in water 4.5-7x1.8-3 oil guttules 1 µm, asci 22-33x5.5-7(7.5) arising from croziers, permanent IKI negative and no apical pore is observed, glassy parts were KOH10% resistant, IKI negative after KOH and CR positive
Stip
Hans-Otto Baral,
07-03-2023 09:40
Re : Unguiculella eurotioides?
Stip, you studied this specimen also? Yes, the glassy matrix is clearly KOH-insoluble. Then we can perhaps hope for a sequence? Ascus measurements refer to dead asci since I do not see any living ones? The LBs measure 1 µm but the spores are around 2 µm, perhaps Stip you mean 1.8-2.3 µm?
Stip Helleman,
07-03-2023 22:33
Re : Unguiculella eurotioides?
Hoi Zotto,
Yes a part of this collection was send to me
3 µm wide for spore was an exception, several were 2,5
I put it to drying yesterday perhaps I can get a sequence out of it
Cheers Stip