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Edvin Johannesen
This tiny, rather "rough" erumpent asco was found
Reddening yellow apothecia on twig
Edvin Johannesen,
22-09-2022 22:05
These yellow, short-stalked apothecia, which turn reddish brown on drying/handling were growing on a twig from a deciduous tree in Central Norway. Spores ellipsoid-fusiform with mostly two polar guttules (last photo in phase contrast to enhance these), 11-13 x 5-6 micr. Paraphyses multiguttulate. Asci IKI-, no croziers seen. A reddish resin seen around ascus apex, presumably causing the reddening. Bisporella/Calycina?Thanks.
Hans-Otto Baral,
22-09-2022 22:24
Re : Reddening yellow apothecia on twig
Sorry I cannot say. The excipulum structure could help. Photo of an ascus tip in IKI?
Edvin Johannesen,
22-09-2022 22:49
Re : Reddening yellow apothecia on twig
OK, thanks. I will try to provide this.
Edvin Johannesen,
22-09-2022 23:13
Re : Reddening yellow apothecia on twig
Hope this helps. The ectal excipulum looks a bit "messy", but then again I didn't succed in making a clean thin section. (I also include a better photo of paraphyse guttules).
Hans-Otto Baral,
23-09-2022 09:22
Re : Reddening yellow apothecia on twig
Ha, no I see what you have: Hymenoscyphus phiala, a dubious name of a very clear species. It is in my Cudoniella folder. The only thing is: the asci must arise from croziers. Would you mind sending me the collection data?
Edvin Johannesen,
23-09-2022 11:09
Re : Reddening yellow apothecia on twig
How exciting! I will try to find croziers - must admit I may not have searched carefully enough. I will send you an email with the colletion data, for sure.
Thanks a lot!
Thanks a lot!
Edvin Johannesen,
23-09-2022 13:50
Re : Reddening yellow apothecia on twig
Is this sufficient documentation?
Hans-Otto Baral,
23-09-2022 20:33
Re : Reddening yellow apothecia on twig
Yes of course! What now remains is the substrate. I have about 24 collections, all were on Alnus. If you could try a radial section for the ladder-shaped perforations this could be fixed. Was there any Alnus?
Edvin Johannesen,
23-09-2022 20:42
Re : Reddening yellow apothecia on twig
Great! I will se what I can do. Not sure if there was Alnus there - could be Alnus incana.
Cheers.
Cheers.
Stefan Jakobsson,
23-09-2022 20:55
Re : Reddening yellow apothecia on twig
I also recently collected a specimen which could be H. phiala, probably on Alnus. However, the apothecial base turns blue in IKI. That is a feature I did not find mentioned for H. phiala but it could fit H. imberbis. Is it still phiala?
Hans-Otto Baral,
23-09-2022 21:11
Re : Reddening yellow apothecia on twig
Your IKI is not visible, are the asci really inamyloid? I consider H. phiala possible, but the inner parts of the excipulum should be thick-walled, almost like in Bisporella (now Bispora) or Calycina citrina. You have no photo of the amyloid base? I would not exclude such possibility that H. phiala may react blue at the stipe base - this is easily overlooked.
Edvin Johannesen,
23-09-2022 21:16
Re : Reddening yellow apothecia on twig
I just checked my specimen and I did not see any blue IKI reaction in the stem base. However, I did observe a bluish coloration in the stem base (without adding IKI) in the stereo microscope. See photo.
Stefan Jakobsson,
23-09-2022 21:27
Re : Reddening yellow apothecia on twig
I tried several times to get a blue ascus reaction with IKI but failed to do so.
On the second photo in the lower right hand corner is a bluing stipe base.
Hans-Otto Baral,
23-09-2022 21:50
Re : Reddening yellow apothecia on twig
Of course, sorry, I overlooked. The thick-walled excipular cells I see on Edvin's photos.
Stefan Jakobsson,
23-09-2022 22:17
Re : Reddening yellow apothecia on twig
It seems I have no clear images of the excipulum. Here the best I could find.
Hans-Otto Baral,
24-09-2022 09:37
Re : Reddening yellow apothecia on twig
Yes, this would fit quite well although it dies not show clearly thick walls (perhaps because only surface cells are seen).