16-07-2025 17:34
Bernard Declercq
Hello,I have trouble distinguishing above mention
14-07-2025 11:20
Michel Hairaud
Bonjour, Voici une espèce de (?) Hyaloscyphace
16-01-2023 21:31
Riet van Oosten
Hello, Nearby the find of Calycina claroflava on
14-07-2025 17:55
Yanick BOULANGERBonjourAutre dossier laissé en suspendJe viens de
14-07-2025 11:17
Yanick BOULANGERBonjourJ'ai un dossier Jackrogersella qui est rest
14-07-2025 15:52
Gernot FriebesHi,I wanted to share this collection on Rubus idae
14-07-2025 13:37
Gernot FriebesHi,do you think this collection could be R. ulmari
Lactophenol Cotton Blue
Malcolm Greaves,
28-08-2022 11:04
I use this for staining the warts on Scutellinia and also when heating spores to see if the outer surface loosens. I have just spotted that one bottle I have is LCB but another is just Cotton Blue in Lactic Acid with no mention of the Phenol. Does anyone know what component of the stain causes the loosening?Thanks
Mal
Viktorie Halasu,
28-08-2022 11:44
Re : Lactophenol Cotton Blue
Hi Mal,
I usually use small amount of LACB to not overstain the preparate. When I suspected a species with loosening perisp. but it didn't show it even after gentle heating, I added a drop of lactic acid and then it loosened all right (after more heating or without, I don't remember). Pure LA is very viscous and goes rather slowly into the solution, I usually have to lift the edge of the coverslip a bit to help it overflow the section. And S. mirabilis preparate was not heated at all, but I left it overnight and the perisp. loosened too. I don't have any exper. with phenol, though.
Have a nice sunday.
Viktorie
I usually use small amount of LACB to not overstain the preparate. When I suspected a species with loosening perisp. but it didn't show it even after gentle heating, I added a drop of lactic acid and then it loosened all right (after more heating or without, I don't remember). Pure LA is very viscous and goes rather slowly into the solution, I usually have to lift the edge of the coverslip a bit to help it overflow the section. And S. mirabilis preparate was not heated at all, but I left it overnight and the perisp. loosened too. I don't have any exper. with phenol, though.
Have a nice sunday.
Viktorie
Malcolm Greaves,
28-08-2022 22:36
Re : Lactophenol Cotton Blue
Thanks Viktorie