10-06-2026 13:41
François Freléchoux
Bonjour à nouveau, Voici une trouvaille d'hier.
09-06-2026 18:32
Camille MertensSur morceau de roseau immergé 0,5 - 0,7 mm de dia
10-06-2026 11:53
Steve ClementsBonjour, This disco is abundant on dead stems of
10-06-2026 12:54
Steve ClementsBonjour encore, Pouvez-vous m'aider, s'il vous pl
10-06-2026 10:45
François Freléchoux
Bonjour à nouveau, Encore une détermination qui
08-06-2026 10:16
Spooren Marco
I don`t have a clou about this fungus,it is not in
10-06-2026 09:24
François Freléchoux
Bonjour, J'imagine que cette détermination ne do
08-06-2026 17:00
François BartholomeeusenGood day everyone, On June 5 2026, I collected de
07-06-2026 15:10
William Slosse
Hello everyone,On 05-06-26, I found following asco
Lactophenol Cotton Blue
Malcolm Greaves,
28-08-2022 11:04
I use this for staining the warts on Scutellinia and also when heating spores to see if the outer surface loosens. I have just spotted that one bottle I have is LCB but another is just Cotton Blue in Lactic Acid with no mention of the Phenol. Does anyone know what component of the stain causes the loosening?Thanks
Mal
Viktorie Halasu,
28-08-2022 11:44
Re : Lactophenol Cotton Blue
Hi Mal,
I usually use small amount of LACB to not overstain the preparate. When I suspected a species with loosening perisp. but it didn't show it even after gentle heating, I added a drop of lactic acid and then it loosened all right (after more heating or without, I don't remember). Pure LA is very viscous and goes rather slowly into the solution, I usually have to lift the edge of the coverslip a bit to help it overflow the section. And S. mirabilis preparate was not heated at all, but I left it overnight and the perisp. loosened too. I don't have any exper. with phenol, though.
Have a nice sunday.
Viktorie
I usually use small amount of LACB to not overstain the preparate. When I suspected a species with loosening perisp. but it didn't show it even after gentle heating, I added a drop of lactic acid and then it loosened all right (after more heating or without, I don't remember). Pure LA is very viscous and goes rather slowly into the solution, I usually have to lift the edge of the coverslip a bit to help it overflow the section. And S. mirabilis preparate was not heated at all, but I left it overnight and the perisp. loosened too. I don't have any exper. with phenol, though.
Have a nice sunday.
Viktorie
Malcolm Greaves,
28-08-2022 22:36
Re : Lactophenol Cotton Blue
Thanks Viktorie