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Mot de passe perdu? S'inscrire

30-07-2025 20:52

apo on Pteridium aquilinum
Bohan Jia

Hi together,  I've been looking posts in AscoFra

30-07-2025 10:12

Asco1 sur crotte de mouton
Bernard CLESSE Bernard CLESSE

Bonjour à toutes et tous,Pourriez-vous m'aider à

31-07-2025 16:32

Carbophilous discomycete - Anthracobia pseudorehmii?
Andreas Gminder Andreas Gminder

Dear collegues,today I found on a very fresh fire

30-07-2025 18:06

Orbilia dryadum?
Stefan Jakobsson

On a decorticated twig of Alnus incana on moist so

29-07-2025 18:59

Ayuda con esta
Castillo Joseba Castillo Joseba

Me mandan el  material seco de Galicia, España,

28-07-2025 12:34

Capronia
Enrique Rubio Enrique Rubio

Hi to everybody.I would appreciate any ideas about

28-07-2025 04:59

identification?
Richard Droker

Growing on resin (over moss on Tsuga heterophyll)l

13-06-2025 09:41

Aquatic ascomycete.
Josep Torres Josep Torres

Hello.A cerebriform ascomycete sprouting scattered

13-06-2025 16:34

Strossmayeria-like on Fagus
Andgelo Mombert Andgelo Mombert

Bonjour,Un petit discomycète qui me résiste. Il

24-07-2025 19:02

Discomiceto 2510
Castillo Joseba Castillo Joseba

De esta mañana en tronco caido de Haya A ver si

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Calycellina spiraeae spores
Chris Yeates, 21-10-2021 13:26
Chris Yeates
Bonjour tous

While processing images of this delightful little fungus I noticed that some of the ascospores show a distinct hyaline "sheath". I dismissed the possibility that it was an artefact, largely because it does not show on all the spores photographed in exactly the same way. As you can see it is also evident in the spore seen "end on".

I can easily see that this might be missed by normal light microscopy but wondered whether any other members have noticed this phenomenon?

Cordialement, Chris
  • message #70392
Hans-Otto Baral, 21-10-2021 17:13
Hans-Otto Baral
Re : Calycellina spiraeae spores
Yes, this is a sheath, and it is also visible without DIC :-)

Perhaps the spore surface staines lilac in CRB, which is due to a gel below this sheath.
Enrique Rubio, 21-10-2021 17:37
Enrique Rubio
Re : Calycellina spiraeae spores
I think that these ephemeral sheaths are very frequent in the ascopores just ejected from the ascus in many Helotiales. What happens is that unless they are very conspicuous they go unnoticed.
Chris Yeates, 21-10-2021 20:23
Chris Yeates
Re : Calycellina spiraeae spores
Thank you both for these comments.

Chris
Hans-Otto Baral, 21-10-2021 20:51
Hans-Otto Baral
Re : Calycellina spiraeae spores
Strange is that they are no always seen in a species. Just in C. spiraeae I see them in ERD 4803 but not in ERD 5959. The mentioned lilac stain with CRB seems specific for Calycellina (and some distantly related genera), but absent, e.g. in Calycina. For C. spiraeae, however, I see I once tested CRB and it was negative. But whether this is a good Calycellina is not sure (no sequence).
Enrique Rubio, 21-10-2021 20:59
Enrique Rubio
Re : Calycellina spiraeae spores
What concentration of CRB do you use to try to stain the sheaths?
Hans-Otto Baral, 21-10-2021 21:55
Hans-Otto Baral
Re : Calycellina spiraeae spores
I cannot say. There is no high concentration necessary, The medium may look hyaline but the gel on typical Calycellina spores attracts the dye.
Enrique Rubio, 24-10-2021 09:30
Enrique Rubio
Re : Calycellina spiraeae spores
Thanks, Zotto.