Asco on soil - Forum ASCOFrance

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Asco on soil

Robin Isaksson, 19-10-2020 22:04

Found on soil sling with mosses.

Asci lugols negative. Spores 11-14x5-7 um
Have no clue where to look.
Somehe knows it?

Regards

Hans-Otto Baral, 19-10-2020 22:17

Re : Asco on soil

Uff, could you send more pics of the spores? And make a test: do the drops in the spores disappear in KOH?

This sounds like a Bryorbilia (Orbiliomycetes), a genus for which only one collection is known so far!

Is this a very sparse sample?

Robin Isaksson, 19-10-2020 22:54

Re : Asco on soil

Hi!

Here come pictures of the spores. Sorry about quality.

It seems like the drops remains in koh 10%.
Last picture in koh.
Tested koh+lugols too now and it is no reaction in asci.

There is around 10 apotecia in the collection, i can collect moore because it was plenty of it in the slope.
But it has been cold now 3 days with minus degrees.

Regards

Hans-Otto Baral, 20-10-2020 08:43

Re : Asco on soil

Ah, this would be good if you collect further apos. Please dry them carefully and send them to someone who does sequencing, perhaps Guy Marson. From where is the sample, is the geology acidic?

Robin Isaksson, 20-10-2020 20:42

Re : Asco on soil

Hi,

ok i will collect more on friday.
Its from Sweden, provins Småland. was found on a pile of earth but I have a hard time believing that there would be lime in the ground because everything around was silicate rocks sticking up. we have very little land with lime where I live. so yes its acidic.

I am happy to send a collection to someone who wants it for sequencing. do you have an email to some?

all the best

Hans-Otto Baral, 20-10-2020 20:55

Re : Asco on soil

O.k., thanks! The only Bryorbilia collection so far known was also on silicate but from Asturias.

I hope I can tell you soon an address.

Hans-Otto Baral, 21-10-2020 11:00

Re : Asco on soil

Yes, Guy would like to sequence it. Please let me know your e-mail then I send you his address.

Guy says: please not as registered mail - and the material should be dried beforehand on a "small heat source" (e.g. near the transformer of an electrical appliance or on a radiator).

Robin Isaksson, 21-10-2020 21:07

Re : Asco on soil

hi,

Great!

robinisaksson69(at)gmail.com

Hans-Otto Baral, 10-11-2020 20:58

Re : Asco on soil

For all a good news: sequencing was successful and this species can be assigned to Bryorbilia arenicola, though with 8 nt deviation in the ITS1 but none in ITS2 and LSU D1-D3 (ITS distance 1-1.2%). Unnamed environmental strains of Bryorbilia spp. and other genera are very difficult to align in the ITS and differ by min. 8% in the LSU.

Robin Isaksson, 10-11-2020 22:25

Re : Asco on soil

Thank you for the help with this one!

Regards,

Robin

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