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Lachnum cf. crystallinum
Andrés Valverde Valera, 30-04-2020 11:47
Andrés Valverde ValeraBonjour, 
Je vous envoie ces images d'un échantillon de Lachnum, trouvé sur une brindille de Corylus, dans une forêt humide des Pyrenées catalanes. 
Coupe 0,8-0,9 mm diamètre. Pied 0,5-0,6 mm d'haut. 
Asques 34,8 – 40,8 x 5,1 – 5,5 µm.
Spores 7,7 – 10 x 1,5-2,7 µm.
Poils avec incrustations, légèrement élargis au sommet, 25,7-28,2 x 2,6-3,3 µm.
Paraphyses terminées en point, 53,7-53,9 x 3,7-3,9 µm.
Je pense en Lachnum crystallinum, mais je ne suis pas sûr du tout.
Merci d'avance pour votre aide, 

Andrés
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Lothar Krieglsteiner, 30-04-2020 12:01
Lothar Krieglsteiner
Re : Lachnum cf. crystallinum

Hello Andres,


your Lachnum (s.str.) cannot be L. crystallinum (former Dasyscyphella crystallina) because of the hairs that are granulate to the top. Further, this species grows on Quercus only (?).


Which Lachnum you have found is difficult to say, because we for instance do not know if there are croziers at the base of the asci.


As I see, the paraphyses do not contain (many) vacuoles, and there are no crystals on the top of the hairs.


Best, Lothar

Andrés Valverde Valera, 30-04-2020 12:51
Andrés Valverde Valera
Re : Lachnum cf. crystallinum
Hello Lothar, 
Thank you very much for your answer.
I add two more photos, of the squash of the asci. Luckily I think I could have seen some croziers... and a paraphyse with more vacuolar content? 

Best, 
Andrés 

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Hans-Otto Baral, 30-04-2020 14:11
Hans-Otto Baral
Re : Lachnum cf. crystallinum
Hi Andrés

indeed you have a Lachnum species with multiguttulate paraphyses (the vacuolar bodies are very sensitive and disappear upon presure or when mounting in KOH etc.).

I see a slight reddening of the apos on your macro, this is due to the presence of these VB-guttules that easily get oxidated.

The circle around the ascus base shows rather a simple-septate ascus, but it could be hidden behind.

If the hairs would be more capitate I tend to L. pudibundum. Are the hairs really that short?
Andrés Valverde Valera, 30-04-2020 16:20
Andrés Valverde Valera
Re : Lachnum cf. crystallinum
Thank you very much Zotto! 
About the hairs, it's the length that I could measure, from the top of the hair to the most dense and colored part (I can't ruled out that they will continue in this part). I'm going to try to separate these hairs. 

Andrés