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Arnium
matthaeus koncilja, 07-04-2020 09:13
Hello all,
I would like to identify a fungus, that I found on rabbit dung. I have observed some features and I think that the fungus belongs to Arnium, but I am not sure. There are also similar genera, par ex. Podospora (without pedicel).

Perithecia pyriform 500-600 x 350-400 mcm.
The perithecial neck is covered with rigid brown polyseptate isolated hairs, up to 160 mcm long.
The outer side of the perithecium is covered with thin brown hairs.
The 8-spored, more or less longstalked asci have equilateral biseriate spores. I cannot say, how many germ pores there are. I suppose, that there are 2. I cannot clear 100% this feature.
The spores with polar gelatinous caudae (one at each end) appears to be without pedicel.
Spore size: (35) 36 – 40 (43) x 20 – 23 (30) mcm.
In cotton blue the apical ring of the asci is not visible, I think it is absent.
The eccentric caudae are thickwalled, from 50-100 mcm long, but often ruptured, so they appear shorter. It seems, that they have a middle channel (?).The caudae are very difficult to view.


Following the Doveri's key I came to Arnium hirtum, on condition that the asco is a Arnium and the asci are without apical ring and the spores have 2 germ pores.
Arnium cervinum is described as similar, but it occurs only on cervine dung.


I would be very happy for any suggestion how to stain the slide preparation for making visible the shape and form of the caudae and the spore germ pores.


Thank you for your help!


matthaeus

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Michel Delpont, 07-04-2020 16:23
Michel Delpont
Re : Arnium
Hello Matthaeus!

Nice find! Compare with A.cervinum and try to observe the existence or not of the germ pores and whether or not the appendages cover them.


Michel.

matthaeus koncilja, 07-04-2020 18:44
Re : Arnium
Hello Michel,

thank you for the proposition. I will try again.

The problem is, that the caudae will not stain in any color I have.

I think, that the germ pores are visible on the last foto I added.

matthaeus
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matthaeus koncilja, 08-04-2020 08:39
Re : Arnium
Hello Michel,

You are right, Arnium cervinum goes better than A. hirtum.

Doveri does not describe in his monograph A. hirtum, but he mention in his observations of A. cervinum, that A. hirtum has centrally arranged appendages. A. cervinum has eccentrically arranged appendages. In A. cervinum the appendages are solid, in A. hirtum hollow.

In my find the spores are in some cases slightly inequilateral, but in the majority of cases they are equilateral. The germ pore is mostly not covered by the appendage.

The appendages are clearly eccentric. Infortunatly I cannot show in appropriate way the appendages. This must be due to the law concentration of my chlorazole. Now I am also for Arnium cervinum.

Thank you again for your help!

matthaeus
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