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dark brown apothecia, splitIKI-Spores biguttulate

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Ascomycete on Magnolia
Gernot Friebes, 24-01-2019 13:59
Hi,

we need your help with this curious ascomycete on twigs of Magnolia. Here are a few key characters:

-) Ascomata glabrous, non-stromatic, sitting rather loosely on/between conidiophores of a hyphomycete (asexual morph of the same species?)
-) Asci apparently bitunicate, reaction strongly to IKI, maybe with a slight amyloid reaction of the (inner?) wall
-) Ascospores faintly striate, 12.5-14 x 4.3-5.2 µm

Thanks for any ideas!

Best wishes,
Gernot
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Viktorie Halasu, 24-01-2019 14:44
Viktorie Halasu
Re : Ascomycete on Magnolia
Dear Gernot,

Letendraea helminthicola looks a lot similar (based on Ellis & Ellis). I don't know what other species are in this genus.

Viktorie
Peter Wilberforce, 24-01-2019 15:52
Re : Ascomycete on Magnolia

Hello Germot,


I acompletely agree with Viktone that this is Letendraea. Ihave two collections of this seemingly rare ascomycete: once from Cumbria and recently from the gerden here in Argyll


Peter

Gernot Friebes, 24-01-2019 16:11
Re : Ascomycete on Magnolia
Hello, Viktorie & Peter,

thanks a lot for your help! I think the genus is pretty clear now indeed. I'm still a bit irritated by the faintly striate ascospores, however. After quickly reading through some descriptions of L. helminthicola I didn't find this feature mentioned, in fact two sources (Dennis in British Ascomycetes and Akulov 2015) explicitly write "smooth" regarding the spore surface. If anyone has further insight into this I would really appreciate it.

Best wishes,
Gernot
Viktorie Halasu, 24-01-2019 23:29
Viktorie Halasu
Re : Ascomycete on Magnolia
Hello Gernot,
could the ornamentation be soluble in some reagent, e.g. KOH, MLZ? Or either soluble or unstainable in LACB?
edit: Akulov used 3% KOH.
Viktorie
Gernot Friebes, 24-01-2019 23:40
Re : Ascomycete on Magnolia
Hello, Viktorie,

I would have to check that. For now all I can say is that the striation is very faint but still well visible in water; both myself and the person who collected the material observed it independently of each other. But if one doesn't look carefully it could certainly be missed.

Best wishes,
Gernot
Gernot Friebes, 28-01-2019 22:52
Re : Ascomycete on Magnolia
Among the Helminthosporium we also observed these tiny conidiomata with conidia measuring about 7.8–8.3 x 4.0–4.3 µm. Do you have any idea to which species they might belong?

Best wishes,
Gernot
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