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Propolis viridis?
Miguel Ángel Ribes, 15-08-2008 01:21
Miguel Ãngel RibesI am going to try it again with an easier fungi, I hope.

On Eucalyptus globulus wood, 2-3 x 1 mm, green-blue color, broken the wood. Gelatinous flesh. I think it is near P. viridis, according to Baral (sporal size and shape, paraphysis with flexuous apex, inamiloid asci with a red-brown mass inside, and bluish colour). But in the CABI the actual name is P. farinosa (Pers.) Fr. with a lot of sinonyms like P. versicolor Fr. and P. viridis Dufour. Everytimes I have see P. versicolor it was white, but it is described with several colors. Is there one or two species?

Sporal measures (1000x, in water and fresh material)
21.4 [23.5 ; 24.6] 26.7 x 8.3 [9.6 ; 10.3] 11.6
Q = 1.9 [2.3 ; 2.5] 2.9 ; N = 24 ; C = 95%
Me = 24.09 x 9.98 ; Qe = 2.43

Thank you
  • message #5282
Miguel Ángel Ribes, 15-08-2008 01:21
Miguel Ãngel Ribes
Re:Propolis viridis?
Another closer photo
  • message #5283
Miguel Ángel Ribes, 15-08-2008 01:23
Miguel Ãngel Ribes
Re:Propolis viridis?
8 spores and inamiloid asci in IKI
  • message #5284
Miguel Ángel Ribes, 15-08-2008 01:24
Miguel Ãngel Ribes
Re:Propolis viridis?
8 spores asci in red congo
  • message #5285
Miguel Ángel Ribes, 15-08-2008 01:25
Miguel Ãngel Ribes
Re:Propolis viridis?
Paraphysis branched and flexuous in red congo
  • message #5286
Miguel Ángel Ribes, 15-08-2008 01:26
Miguel Ãngel Ribes
Re:Propolis viridis?
Mature spores in red congo
  • message #5287
Miguel Ángel Ribes, 15-08-2008 01:29
Miguel Ãngel Ribes
Re:Propolis viridis?
Spores in water
  • message #5288
Hans-Otto Baral, 15-08-2008 15:28
Hans-Otto Baral
Re:Propolis viridis?
Hi Miguel
as you can see from my DVD, there is rather strong variation among the finds I refer to P. viridis. Spore length ranges from 14-19 up to 22-31 µm. But those large-spored are uncertain, and mediterranean viridis ranges (14-)16-22(-26) x 5.5-8.5 µm. The apos may somtimes be only greenish at the margin, or even entirely white!

So what you have here is unclear to me. At least I am sure that your photos show dead spores. Why? You say the measurements are made from fresh in water? The photos not? The oil drops should be much more distinct, and the plasma not detached from the wall. Looks like in lacophenol. Propolis is xerotolerant (like Srictis), so you can rehydrate the apos many weeks later and have living spores to study, perhaps over a period of half a year.

Zotto
Miguel Ángel Ribes, 16-08-2008 12:16
Miguel Ãngel Ribes
Re:Propolis viridis?
Hi Zotto

I don't know why my spores are dead cause photos macro are made at 18/06/2008 and photos micro at 29/06/2008, only 11 days after, and all this time the species has been inside a fridge. The spores measuements are made with Piximetre 3.8 over photos micro in glicerine water, only free spores. I am sending more spores photos in water in order to see if are they dead¿¿??

I have made a new measurement, this time with 400x photos in glicerine water, and the results are very similar:
Sporal measures (400x in water, fresh material)
20.1 [23.1 ; 24.7] 27.7 x 8.4 [10.3 ; 11.2] 13.1
Q = 1.6 [2.1 ; 2.4] 2.9 ; N = 25 ; C = 95%
Me = 23.89 x 10.74 ; Qe = 2.25

Thanks Zotto, you are very patient
  • message #5298
Miguel Ángel Ribes, 16-08-2008 12:16
Miguel Ãngel Ribes
Re:Propolis viridis?
Anothe spores in glicerine water
  • message #5299
Miguel Ángel Ribes, 16-08-2008 12:18
Miguel Ãngel Ribes
Re:Propolis viridis?
And another one in water with 1000x
  • message #5300