Geoglossum - Forum ASCOFrance

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Geoglossum

Malcolm Greaves, 26-10-2015 15:31

This was found alongside a Geoglossum hirsutum. With almost no stalk and over 11cm tall it is the largest one I have found. Spores hyaline when immature with 1 - 7 septa, dark with 7 spepta when mature 70 x 6µ. Paraphyses swollen and mostly bent at the top.
Is this G umbratile.
Thanks
Mal

Hans-Otto Baral, 26-10-2015 15:44

Re : Geoglossum

I would look in water for living asci whether they are brown or hyaline. G. fallax could be an option, and this species ejects hyaline spores, contrary many others.
Zotto

Malcolm Greaves, 26-10-2015 18:20

Re : Geoglossum

None of the ejected spores look hyaline. In the range 55-65 and 3-7 septate (almost all 7).
Mal

Hans-Otto Baral, 26-10-2015 22:34

Re : Geoglossum

o.k., but if you have only the dried fungus then it is difficult to get a clue about this. If many asci contain brown spores then you can assume that threy are ejected when already brown.

Malcolm Greaves, 26-10-2015 23:58

Re : Geoglossum

Still fairly fresh Zotto.
Mal

Hans-Otto Baral, 27-10-2015 08:49

Re : Geoglossum

Then please try a preparation in water without pressure on the cover slip. It is easy to distinguish between living and dead asci. As soon as you see one living (turgescent) ascus that contains brown spores you can exclude G. fallax. I am not very familiar in Geoglossum, perhaps others can suggest a name for your fungus.

Malcolm Greaves, 27-10-2015 15:26

Re : Geoglossum

Mounted in water all the acsii seem to show dark spores so I think G umbratile seems a good fit.
Thanks for your comments Zotto.
Mal

Sabino Arauzo, 27-10-2015 15:36

Re : Geoglossum

Hi Malcolm, Zotto:

The morphology of the spores (guttules) and paraphyses is not G. fallax.

The stipe looks smooth, fit into our concept of G. umbratile.

Best regards.

Sabino Arauzo.

Hans-Otto Baral, 27-10-2015 16:47

Re : Geoglossum

The ascus wall is not visible, so I cannot decide whether the asci are alive or not. But with so many brown spores I would also exclude fallax.

And yes indeed, in G. fallax I saw the spores with large drops with some small around, but here they are multiguttulate.

In your work, Sabino, G. nigritum and G. umbratile, are synonyms, and you figured a multiguttulate spore. But my cf. umbratile HB6543 has gutules like fallax and also hyaline spores inside the asci. I compared it otherwise with G. (Hemileucoglossum) elongatum.

Zotto

Sabino Arauzo, 28-10-2015 12:39

Re : Geoglossum

Hi Zotto:

I have not seen amorphous brown material in paraphyses of G umbratile or closest species. H. elongatum has ephitecium and stipe hairy.

I think that HB6543 belongs to G. fallax - starbaeckii group.

Sabino.

Hans-Otto Baral, 28-10-2015 12:48

Re : Geoglossum

Thanks, this sounds reasonable, since the stalk was strongly floccose there.

I only wonder why in my HB 1217 no exudate is shown, perhaps I overlooked.

Zotto

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