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Malcolm  Greaves Malcolm Greaves

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Is anyone with experience of DNA testing able to t

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Peziza muscicola or berthetiana?
Carmel Sammut, 04-02-2015 22:01
Hi,
   I have a few collections of a black Peziza from the same area collected over the last 2 years. For now I am presenting this specimen which has some characteristic of P. muscicola. The specimens were collected (10/1/15) amongst mosses in an area with Pinus halapensis, at Verdala Buskett, Malta.
The collected material was 15-30 x 14-23mm, hymenium dark black, inner surface smooth sometimes pitted. No latex was observed on cutting or breaking. Asci J+ve, biseptate. Paraphyses slightly inflated, septate. Spore are biguttulate at the polar ends, very rough with ?ridges, reticulum, warts - after staining with lactophenol CB. Size (11.5) 12.8 - 16.8 (17.3) x (7.4) 8.1 - 9 (9.2)µm Q = (1.3) 1.4 - 1.9 (2); Me = 14.6 x 8.7 µm ; Qe = 1.7. Other collections show slightly larger spores up to 18µm in lenght.
What is your opinion?
 
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René Dougoud, 06-02-2015 09:47
Re : Peziza muscicola or berthetiana?
Cher Collègue,

Il convient cependant de définir la composition de la chair. Selon Hohmeyer la chair de P. muscicola  se compose : d'un exc. méd, sup. de t. intricata ; d'un exc. méd. moy. de t. globulosa ; d'un exc. méd. inf. de t. intricata ; d'un exc. ectal de t. angularis.

Chez P. berthetina, l'exc. méd. sup. de t. intricata ; exc. inf. de t. globulosa ; exc. ectal de t. angularis. Surtout les asques son aporhynques, soit sans crochet (Donadini 1984, Doc. Myc. 56 : 39-47)

Cordialement

René
Carmel Sammut, 08-02-2015 20:04
Re : Peziza muscicola or berthetiana?
Thank you René for your detailed explanation. The medullary excipulum appears globular, but I did not examine the intermediate layers. I will take a look when I find some time. The asci are of the pleurorhyncus type, at least that is easier to find.