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Cercophora sp.?
Stefan Blaser, 27-05-2013 10:04
Hello everybody

I hope someone can help me with this Cercophora(??) species.


I found it on very clayish soil at a disturbed site without obvious connection to dung or wood.


The Perithecia are brown to nearly black with apressed brown hairs on the outside. The spores, when immature, are greenish/brownish about 50-60 x 4-5 µm with hyaline, pointed appendages, variably and often sharply bent. Appendages get quite weird, when Spores are getting more mature (see picture). I guess I did not have fully mature Spores, as Perithecia startet to decay instead of maturing. But the most mature Spores had a somewhat lanceolate darker brown end with an additional Septum in the dark part, about 16x5 µm.


It would be great to get an Identification and if available also some Literature on the Species /Genus


Thanks for any help


Stefan

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Hans-Otto Baral, 27-05-2013 10:11
Hans-Otto Baral
Re : Cercophora sp.?
Hi Stefan

I once saw something similar, which Jacques Fournier identified as Eosphaeria uliginosa (he has also a part of the specimen). But the straight part of the spores does not swell when turning dark brown.

I do not understand how it is possible to separate  a genus Eosphaeria from Cercophora.

Zotto
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Stefan Blaser, 27-05-2013 10:17
Re : Cercophora sp.?
Hi Zotto

This seems to be quite close to my collection. I have to take a closer look at the peridium this evening where these clods should be visible. 

Thanks a lot
Jacques Fournier, 27-05-2013 10:26
Jacques Fournier
Re : Cercophora sp.?
Hi Stefan,
indeed E. uliginosa, with its  unusual habitat on soil. Your photo 4 is very interesting because we can see overmature spores yielding phialides and conidia directly, a feature sometimes encountered in Lasiospaheriaceae.
The swelling of the head of spores, their septation and pigmentation are quite variable in this species and Andrew Miller has shown that the delimitaion of Cercophora based on swelling and darkening of the head is artificial.
I send you in private the paper on Eosphaeria.
Cheers,
Jacques
Hans-Otto Baral, 27-05-2013 10:38
Hans-Otto Baral
Re : Cercophora sp.?
Hi Jacques

Do you mean  the paper by Miller & Huhndorf 2004? I have only a few pages, can you send it me also? 

E. uliginosa is described in detail by Petrini et al. 1984, Trans. B. Mycol. Soc. 82: 554-556

Zotto
Stefan Blaser, 27-05-2013 10:40
Re : Cercophora sp.?
Hi Jacques

Great! Thanks a lot for the ID and the Paper.

Stefan
Andrew N. Miller, 27-05-2013 12:55
Andrew N. Miller
Re : Cercophora sp.?
All of my publications can be found and downloaded here as PDFs:  http://wwx.inhs.illinois.edu/research/pi/amiller/publications

Stefan, I have been looking for this species for over 15 years now.  It is rarely collected.  It would be very interesting to include in my phylogney of Sordariales.  Would you be interested in sending me 25-30 ascomata to use in DNA sequencing? 

Thank you,
Andy
Stefan Blaser, 27-05-2013 13:48
Re : Cercophora sp.?
Hi Andy

Unfortunately, my material is in a rather poor condition now. I will try to find some fresh Material at the collection site today. I will tell you if I was lucky...

Stefan
Andrew N. Miller, 27-05-2013 13:56
Andrew N. Miller
Re : Cercophora sp.?
Thank you!  My colleagues have found this species before a couple of times, but also not in good condition or few ascomata.  Someday I hope to be able to conduct molecular studies on this species (and all cryptic species...;o).

Best,
Andy
Stefan Blaser, 28-05-2013 18:32
Re : Cercophora sp.?
Hello everybody

I think I have to "reopen" this thread. I did find fresh material in the field and I took a closer look at the Perithecium anatomy. I find a wall that consists of globose brownish cells. On the wall surface were bundles of brown, slightly thickwalled hairs arising from wall cells. For me, this does not at all fit with the description of Eosphaeria uliginosa in Petrini&Petrini. I do not find "scutate" structures and hairs are not mentioned there. For details see photos.


So what now?


Stefan


?

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Andrew N. Miller, 28-05-2013 20:37
Andrew N. Miller
Re : Cercophora sp.?
What do the spores look like?

Andy
Stefan Blaser, 28-05-2013 20:48
Re : Cercophora sp.?
Hi Andy

Like in the pictures at the start of the thread. Spores with dark brown end cells like in pic6 were only observed in the old material but not in the fresh one I collected yesterday. Either very mature or overmature ones. However a slight swelling on one side is visible in several spores.

Stefan
Stefan Blaser, 28-05-2013 20:59
Re : Cercophora sp.?
Some more pictures...
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Andrew N. Miller, 29-05-2013 01:12
Andrew N. Miller
Re : Cercophora sp.?
I guess it is entirely possible that you have a mixed collection of an Eosphaeria sp. and a  Cercophora sp., but I would need to examine the specimens to be sure.

Andy