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02-07-2025 09:32

Nicolas VAN VOOREN Nicolas VAN VOOREN

Hello, bonjour.Here is the paper I'm searching for

30-06-2025 16:56

Lydia Koelmans

Please can anyone tell me the species name of the

01-07-2025 23:37

Josep Torres Josep Torres

Hello.A Pleosporal symbiotic organism located and

30-06-2025 12:09

Edvin Johannesen Edvin Johannesen

This tiny, rather "rough" erumpent asco was found

30-06-2025 06:57

Ethan Crenson

Hi all, Another find by a friend yesterday in Bro

30-06-2025 19:05

ALAIN BOUVIER

Bonjour à toutes et à tousJe cherche à lire l'a

30-06-2025 14:45

Götz Palfner Götz Palfner

This is a quite common species on Nothofagus wood

25-06-2025 16:56

Philippe PELLICIER

Bonjour, pensez-vous que S. ceijpii soit le nom co

29-06-2025 18:11

Ethan Crenson

Hello all, A friend found this disco yesterday in

28-06-2025 17:10

Peter Welt Peter Welt

I'm looking for: RANALLI, M.E., GAMUNDÍ, I.J. 19

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Hysteropezizella diminuens
Nina Filippova, 12-02-2013 16:44
I saw very beautiful blue coloring of VBs at tips of paraphyses  in Zotto's collection of this species, 9413 (CRB), which i did not succeed to see. Is it vital reaction with cresil blue?

From dead leaves of Eriophorum angustifolium, wet bog hollow.

Apothecia immersed in tissue, ellipsoid, to 350 x 170 mk, opening by ellipsoid slit, disc pale, surrounded by dark brown edge.

Excipulum from textura angularis at base, cells dark brown, about 10 mk in diam, textura prismatica at the edge, end cells (hairs) cylindrical or clavate, 15-20 x 5-8, brown, walls of end cells under immersion with roughness; asci clavate, inamyloid, 59-67 x 12,5-14,6; paraphyses cylindrical with ampulliform end cell, about 3 mk broad and segmented in lower part, ampulliform cell up to 5 mk in broadest part, hyaline, with small guttules (rarely seen in rehydrated), not or some exeeding the asci; spores subfusoid, many-guttulate, 17 (15,7-17,7) x 3,7 (3,4-4,2) (Q=4,67; N=13).
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Hans-Otto Baral, 12-02-2013 18:09
Hans-Otto Baral
Re : Hysteropezizella diminuens
Yes, these are globose, rather inconspicuous (low-refractive) VBs which stain turquoise-blue in Cresylblue, but you see them only in living state.

Did you pretread your asci with KOH?

Hysteronaevia olivacea would have inamyloid asci, and paraph. as yours, but has much broader spores with acute ends.

Zotto

Nina Filippova, 12-02-2013 19:04
Re : Hysteropezizella diminuens
I used water-lugol, and MLZ and they were inamyloid, now added KOH, heated, and strong amyloid reaction happened. So, this shows they are actually amyloid?

Paraphyses hyaline and brownish in different fbs at one leaf, is seems variable.

I posted Hysteronaevia olivacea some before: http://www.ascofrance.fr/forum?page=1&id=21842
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Hans-Otto Baral, 12-02-2013 21:19
Hans-Otto Baral
Re : Hysteropezizella diminuens
This is the very typical hemiamyloid reaction, and it shows that your Lugol is insufficient. You should get a bright red reaction in water + lugol. Maybe your solution is almost colourless (the photo looks so), or it contains unallowed  ingredients. Hemiamyloid rings in Melzer are negative without KOH.

Sorry, I confused your two postings. So yes, this is H. diminuens. My answer on paraphyses, of course, concerned the Hysteronaevia, I will change it accordingly.

Zotto
Nina Filippova, 13-02-2013 11:55
Re : Hysteropezizella diminuens
There is ascus pore in fresh prepared lugol, it is still not pure red, apparently since J crystals not fully dissolved yet and solution is some weaker. But finally, i can see red reaction too.
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Hans-Otto Baral, 13-02-2013 12:09
Hans-Otto Baral
Re : Hysteropezizella diminuens
Yes, this is fine! Possibly the reaction has a blue component that becomes visible at lower iodine concentrations, resulting in a more dirty red (type RB).