I do not know this species, but your pcs and data resemble indeed very much those on Stip's drawing:

Hamatocanthoscypha ocellata, SH 192-1.JPG

Are you sure you had the spores in KOH? They look as if alive!

Zotto

Yes, it look similar i think. Only I had not seen brown pigments in hairs and excipulum, otherwise coinsides. I thought of H. obsoleta since my spores some smaller than in H. ocellata described, but mine not alive.

No, nothing alive under 1 m deep snow). So, used KOH to rewet them, but guttules still saved, inspite Stip writes "KOH scatters the guttules" (probably since solution is weak).

Why shouldn't the spores remain alive under snow? probably they even survive drying for several weeks. How diluted is your KOH? I still think your spore photo shows living spores.

Zotto,

probably i misunderstand, what do you mean by living? They will grow, if there are suitable conditions, e.g. they are dormant (I did not kill them with some chemicals or at flame). But apothecia were collected in september, and were dried. Now i rehydrated them. I am trying to use water for mounts at first, but when tissues are still cohered i add KOH. The solution was prepared in 10% concentration, but later i added more water, so it is some weaker.?

Ah o.k., 10% is a rather high concentration. Concentrations of 1% are already toxic within a few seconds. 0.1% I did not test so far, but even there I fear KOH is toxic to fungal cells within perhaps 10-20 seconds.

To examine collections from summer and autumn during the winter months is convenient, I understand very well. But it is very disadvantageous. I regret I still do not have my powerpoint about this online. In any case you will meet living spores in your herbarium specimens. Usually asci etc. do not survive drying for so long, but in all those species that grow on exposed substrate such as attached branches etc. you can study fully alive apothecia even after 1/2 year or more. Only such species are dormant as a whole. The others have only dormant spores but these survive very different periods of time in the dormant state, between a few days up to several years, irrespective of whether they are hyaline or brown, thin- or thick-walled.

When you rehydrate dry living spores they gain their original shape within a few seconds. Of course, germination would take longer, some hours or so.

Zotto

Ah o.k., 10% is a rather high concentration. Concentrations of 1% are already toxic within a few seconds. 0.1% I did not test so far, but even there I fear KOH is toxic to fungal cells within perhaps 10-20 seconds.

To examine collections from summer and autumn during the winter months is convenient, I understand very well. But it is very disadvantageous. I regret I still do not have my powerpoint about this online. In any case you will meet living spores in your herbarium specimens. Usually asci etc. do not survive drying for so long, but in all those species that grow on exposed substrate such as attached branches etc. you can study fully alive apothecia even after 1/2 year or more. Only such species are dormant as a whole. The others have only dormant spores but these survive very different periods of time in the dormant state, between a few days up to several years, irrespective of whether they are hyaline or brown, thin- or thick-walled.

When you rehydrate dry living spores they gain their original shape within a few seconds. Of course, germination would take longer, some hours or so.

Zotto

Ok,
so as these specimens were stored only 3 months they could be alive the whole, and spores as well. Alive microscopy - i am willing to follow this rule in next season).