The microscophy wasn't exactly, what I expected.
The spores were about 9-12x2-2,5 and could be bent on the "tail".
I think, that thungsten may not be the best choice for cameras lightning setting, when using led lights ;)

Another poor one:

The asci were about 35-40 (-50)x4-5 and paraphyse tips could be swollen up to 2-3 micrometers.

Could this still be O. coccinella or O. vinosa or something I should already know...

Marja

Difficult to say with dead material. Your scale, is it 2 µm per step?

I assume the ascus is hemispherical and thick-walled. Oh no, I see the paraphyses are capitate, so probably the asci are thinwalled and partly truncate. I assume it is O. subtrapeziformis, a species typical of montaneous or boreal areas.

Zotto

Hi Zotto,

thanks :)
I had really hard times trying to determine this. I can take another look later. I saw no spore bodies, so the spores may be dead, maybe some other fruitbodies can reveal something.

Marja

O.k., it seemed to me you have only two tiny ones. If you have more, yes: look for living spores. They should contain a long and narrow spore body.

I asume your photos were made without oil immersion? What's the scale's meaning?

Zotto

Hi Zotto,

I used 500x meaning, that factor 2x should be used with the scale. Oil immersion doesn't help now. The details show better with 500x than 1000x with my poor light condition.
I think I saw some living spores, but can't say a thing about the spore bodies. Inside asci were some narrow lines. I've noticed, that with my microscope spore bodies may be sometimes seen easiest inside the asci. The short tail like seems sometimes to move in water as if the spore was trying to behave like a dog ;)

Hopefully I have this week time to go to the university with the specimen and look there. I've still got maybe 10 fruitbodies left...

Marja

You are right: when free spores are dead then a chane exists that inside the dead asci there are living spores. And as you explain: you have surely seen filiform SBs there. There is a very similar species, O. filiformis, which would be another option. That has very narrow and long SBs. And a different anamorph. Perhaps you find some conidia?

Now I understand your light problem.

Zotto

Ok.

I try to hunt conidias later this evening home. I'll propably go to university on friday.

Marja

Hi,

just returned from a succesful, but confusing hunting.
Most of the catches were small, length under 20 micrometers:

But there were some bigger with 2 legs and then this:

Weather this one is a conidia, I can't say, but suppose, that someone else may say ;)

And all these came from a maybe 2 cm x 1 cm slice of now very, very wet wood.

Marja

Nice! The first is O. eucalypti (formerly coccinella) anamorph Dicranidion fragile. The second is what I was expecting: a Tridentaria, the presumed anamorph of O. filiformis. And the third is well-known to Guy, but we have no name. It is like a "K", similar to a Dwayaangam.

So based on the presence of Tridentaria and the absence of Trinacrium I suspect your teleomorph is O. filiformis. But try to see living spores.

Zotto

Thanks Zotto.

I'll try to see the spores on friday, when I hopefully spend a couple of hours at the university at a better microscope.

Marja