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Nicolas Suberbielle
Bonjour à tous, Je recherche cette publication :
Orbilia on soaked wood
Martin Bemmann,
12-07-2010 21:19
After some time I tried an Orbilia again....I found it today on a piece of rotten soaked wood, lying in a small creek, half submerged.
The few (4-5) apothecia are very fragile and light apricot in colour.
Asci are 8 spored, uni- to biseriate, IKI- and with a furcate base. (28-34.5 x 3.2-39 µm, without base)
Paraphyses are covered at the tip with a gelatinous mass, shape is capitate and there are septa in the stipe.
The spores are ovoid to fusiform featuring a prominent refractive body. (3.2-4.2 x 1.3-2 µm)
I believe that I have found O. coccinella two times before. Is it the third time?
Regards,
Martin
Martin Bemmann,
12-07-2010 21:19
Re:Orbilia on soaked wood
Asci:
Martin Bemmann,
12-07-2010 21:20
Re:Orbilia on soaked wood
Paraphyses:
Martin Bemmann,
12-07-2010 21:20
Re:Orbilia on soaked wood
Spores:
Martin Bemmann,
12-07-2010 21:21
Re:Orbilia on soaked wood
And textura of the excipulum:
Enrique Rubio,
12-07-2010 21:33
Re:Orbilia on soaked wood
It seems to be Orbilia coccinella or related species.
Enrique
Enrique
Michel Hairaud,
12-07-2010 22:15
Re:Orbilia on soaked wood
I have the same idea as Enrique, but that's just ''teasing'' before our Orbilia maestro's answer ... :P
Hans-Otto Baral,
12-07-2010 22:51
Re:Orbilia on soaked wood
Hi Martin & Enrique
a pretty good find! And the spore photo excellent. It shows a laterally oriented, somewhat rod-shaped spore body. Did you look at the margin? The crenulate appearance is because of glassy processes, I am quite sure. So this must be Orbilia orientalis, a species which has an anamorph of the genus Drechslerella which forms constricting rings and large 3-septate cylindrical conidia.
Martin , wenn du die Bilder in höherer Auflösung hast, würde ich sie gerne haben. Teile mir doch bitte auch noch die Funddaten mit. Und wenn du die Holzanatomie anschauen möchtest (besonders Blick auf Querbruch), vielleicht wäre es ein neues Substrat.
Zotto
a pretty good find! And the spore photo excellent. It shows a laterally oriented, somewhat rod-shaped spore body. Did you look at the margin? The crenulate appearance is because of glassy processes, I am quite sure. So this must be Orbilia orientalis, a species which has an anamorph of the genus Drechslerella which forms constricting rings and large 3-septate cylindrical conidia.
Martin , wenn du die Bilder in höherer Auflösung hast, würde ich sie gerne haben. Teile mir doch bitte auch noch die Funddaten mit. Und wenn du die Holzanatomie anschauen möchtest (besonders Blick auf Querbruch), vielleicht wäre es ein neues Substrat.
Zotto
Martin Bemmann,
12-07-2010 23:38
Re:Orbilia on soaked wood
Hallo Zotto,
I will try to meet your demands by tomorrow. There are 2-3 apothecia left. Especially I will try to make a good section to study the margin.
As for the host, my first guess is pinus, but I will try to give you more details, too.
Always good for a surprise, those microfungi... ;-)
Herzliche Grüße,
Martin
I will try to meet your demands by tomorrow. There are 2-3 apothecia left. Especially I will try to make a good section to study the margin.
As for the host, my first guess is pinus, but I will try to give you more details, too.
Always good for a surprise, those microfungi... ;-)
Herzliche Grüße,
Martin
Martin Bemmann,
13-07-2010 17:33
Re:Orbilia on soaked wood
Hi Zotto, Enrique, Michel and whoever might be interested,
I made a section from the same specimen I probed yesterday to avoid a mixup (I saw Zotto had a mixed collection with O. coccinella). It was still attached to the wood and looked fresh. I must apologize in advance for the many pictures to come. I hope some are helpful.
At the margin I found some refractive elongated cells, some of them glued together. They stained in BKB (no picture of that). The pictures are shot through a 10x, 40x and 100x objective.
I made a section from the same specimen I probed yesterday to avoid a mixup (I saw Zotto had a mixed collection with O. coccinella). It was still attached to the wood and looked fresh. I must apologize in advance for the many pictures to come. I hope some are helpful.
At the margin I found some refractive elongated cells, some of them glued together. They stained in BKB (no picture of that). The pictures are shot through a 10x, 40x and 100x objective.
Martin Bemmann,
13-07-2010 17:33
Re:Orbilia on soaked wood
40x
Martin Bemmann,
13-07-2010 17:34
Re:Orbilia on soaked wood
100x
Martin Bemmann,
13-07-2010 17:34
Re:Orbilia on soaked wood
The exudate at the paraphyses stained blue as well in BKB.
Martin Bemmann,
13-07-2010 17:35
Re:Orbilia on soaked wood
Again some spores (in BKB) :
Martin Bemmann,
13-07-2010 17:35
Re:Orbilia on soaked wood
and with oblique illumination:
Martin Bemmann,
13-07-2010 17:36
Re:Orbilia on soaked wood
On the surface of my section I found some conidia(?) with 3 septa that might be of interest:
Martin Bemmann,
13-07-2010 17:36
Re:Orbilia on soaked wood
And now for the host...
I have no experience at all with the microscopy of wood and will leave it to you. I made section through the branch and a thin cross section I put under the microscope. Would Acer be an option??? At the place I found it, nearly everything is possible except Abies.
Please let me know what I could do more and/or better.
Best regards,
Martin
I have no experience at all with the microscopy of wood and will leave it to you. I made section through the branch and a thin cross section I put under the microscope. Would Acer be an option??? At the place I found it, nearly everything is possible except Abies.
Please let me know what I could do more and/or better.
Best regards,
Martin
Martin Bemmann,
13-07-2010 17:37
Re:Orbilia on soaked wood
close up
Martin Bemmann,
13-07-2010 22:33
Re:Orbilia on soaked wood
Here is a vertical section of the wood:
