04-10-2024 17:08
Birgit LüthjeI just found a very similar ascomycet on a heap of
03-10-2024 20:56
Margot en Geert VullingsThese orange discs were found in September on mois
01-10-2024 17:02
B Shelbourne• Habitat and macro suggest Chlorociboria.• P
01-10-2024 16:15
Lothar Krieglsteiner.. on deciduous twig, on and near old specimens of
08-09-2024 21:31
B Shelbourne• Stromatised substrate and macro like genus Rut
26-09-2024 17:25
Hans-Otto BaralDoes someone have a pdf of this paper? I have it
• Supported by paraphyses, spores and ascus rings.
• The spores, excipulum, and substrate make me think H. s.s.
• Possibly H. caudatus, based on spores, asci, and macro.
Habitat: On petioles and veins of dead leaves of Salix cf. caprea, possibly on a Betula cf. pendula leaf too (no micro), leaves well decayed, on the top of leaf litter, at the edge of a pond, mixed deciduous woodland, Low Weald, England, mid-September, after rain.
Apothecia: Whitish-yellowish, very small, diameter < 1 mm, short stipitate, receptacle whitish, disc opaquer and more yellowish, eventually plano-convex, margin uneven, relatively indistinct.
Asci: Mature and turgid ~110-125 x 8.5-10 um, cylindrical-clavate, 8-spored, biseriate when turgid, simple septa, rings bb, Hymenoscyphus-type, apex acute-truncate, thickening noticeable when flaccid, collar-like opening.
Spores: Scutuloid, quite variable, constricted in the middle, poles rounded, many smallish LBs in each half, most appearing to coalesce into 1-3 of medium size (in each half), uninucleate and aseptate.
Free spores in water: (13.8) 15.4 - 18.7 (19.3) × (4.2) 4.4 - 5.3 µm, Q = (2.7) 3.1 - 3.7 (4.4), n = 30, mean = 16.6 × 4.8 µm , Q mean = 3.5.
Paraphyses: Narrow cylindrical, ~2-4 um wide, multi-septate, rarely branching towards the apex, Hymenoscyphus-type VBs (apex with many medium-size, globose, dextrinoid VBs), pale yellow/chlorinaceous.
Medullary ex: Possibly textura porrecta (not tangled enough for intricata?), hyaline.
Ectal ex: Textura prismatica, hyaline.
I can imagine this is challenging as I found even distinguishing this species quite hard at first, and I noticed some variance in the spore measurements given. However, Declercq's unpublished key does seem to get me there, once I worked out it is in table 2 (low refractive VBs, usually not reddening, folicolous).
Declercq also describes var. constrictus, with small apos, spores with rounded bases, and no reddening with trauma, which seems to fit my observation. There are sequences available for several samples labelled as H. caudatus, do you know if these support heterogeneity or any subgroups?
There are some Chinese sequences under H. caudatus that seem to be related but not identical, but these are very far from my HB 7588c (KM114539) which matches by 0.2% MZ492984 Hymenoscyphus caudatus strain FeF217 from Poland and is a bit more distant to MZ492985 Hymenoscyphus caudatus strain FeF416.
Disturbing is a sequence that is 100% = FeF217, but uploaded as H. albidus:
JX976998 Hymenoscyphus albidus isolate Ha_10203_(VS) (Gherghel et al. 2012 unpubl., 2010 K.H. Rexer, Fraxinus exc.). Clearly this is far from true H. albidus.
The upload of 7588c was not done by me, it lacks collection data: Tübingen, on Fagus cupules. Spores are slghtly constricted, rather broad. A docu is in my caudatus folder.